ALPL produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 493 amino acids (19-503 a.a.) andhaving a molecular mass of 54.5kDa (Molecular size on SDS-PAGE will appear atapproximately 50-70kDa). ALPL is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
ALPL Mouse
目录号: GP21399纯度: >98%
Alkaline Phosphatase Liver/Bone/Kidney Mouse Recombinant
| 规格 | 价格 | 库存 | 数量 | 操作 |
|---|---|---|---|---|
| 1μg | ¥840.00 | 现货 | 1 | |
| 5μg | ¥2,030.00 | 现货 | 1 | |
| 50μg | ¥16,800.00 | 现货 | 1 |
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Nature641, 529–536 (2025)
Nature628, 630–638 (2024)
Nature632, 686–694 (2024)
Nature618, 1017–1023 (2023)
Nature610, 366–372 (2022)
Cell187(9):2288-2304 (2024)
Cell183(7):1867-1883 (2020)
Science388(6745) (2025)
Science387(6739) (2025)
Science387(6734) (2025)
Cell Research35, 97–116 (2025)
Cell Research34, 683–706 (2024)
Cell Research33, 273–287 (2023)
Cell Research33, 546–561 (2023)
Cell Research33, 904–922 (2023)
Cell Research31, 1291–1307 (2021)
产品描述 Description
产品文档 Product Documents
Purity:>98%
Product Data
Purity
Greaterthan 95.0% as determined by SDS-PAGE.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Shipping Condition
Shipped with Ice Packs.
Synonyms
Alpl;Akp-2; Akp2; ALP; APTNAP; TNAP; TNSALP; HOPS; AP-TNAP; Alkaline phosphatase 2.
Amino Acid Sequence
VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVTAARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTRCNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQLMHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLALDPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQALHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNGPGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYASCIGANLDHCA WAGSGLEHHH HHH.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Biological Activity
Specific activity is > 46,000 pmol/min/ug and isdefined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphateto phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Formulation
ALPL protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
