DCFH2-DA (2',7'-Dichlorofluorescein diacetate) is a cell-membrane-permeable universal oxidative stress indicator. This reagent can be used to detect reactive oxygen species (ROS), nitric oxide (NO), and to assess the overall oxidative stress level[1]. DCFH₂-DA is widely used for monitoring cellular redox processes. DCFH₂-DA itself is non-fluorescent. After entering the cells, DCFH₂-DA is hydrolyzed by cellular esterases to generate 2',7'-dichlorodihydrofluorescein (DCFH), which is then rapidly oxidized to the highly fluorescent product 2',7'-dichlorofluorescein (DCF). The fluorescence signal can be detected by fluorescence spectroscopy with an excitation wavelength of 492nm and an emission wavelength of 515nm[2].
References:
[1] Hans C, Saini R, Sachdeva MUS, et al. 2',7'-Dichlorofluorescein (DCF) or 2',7'-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes. Biomed Pharmacother. 2021 Jun;138:111512.
[2] Cowley PM, Nair DR, DeRuisseau LR, et al. Oxidant production and SOD1 protein expression in single skeletal myofibers from Down syndrome mice. Redox Biol. 2017 Oct;13:421-425.
DCFH2-DA (2',7'-Dichlorofluorescein diacetate)是一种具有细胞膜渗透性的通用氧化应激指示剂，可用于检测活性氧（ROS）、一氧化氮（·NO）及评估总体氧化应激水平，广泛应用于细胞氧化还原过程的监测[1]。DCFH2-DA本身无荧光，进入细胞后会被细胞酯酶水解生成2',7'-二氯二氢荧光素（DCFH），随后迅速氧化为强荧光产物2',7'-二氯荧光素（DCF）。DCF荧光信号可通过荧光光谱检测，激发光波长为488nm，发射光波长为525nm[2]。