(Z-Ala-Ala-Ala-Ala)2Rh110 is a sensitive, non-fluorescent elastase substrate. Upon selective cleavage by elastase, the colorless (Z-Ala-Ala-Ala-Ala)2Rh110 yields the strongly fluorescent compound Rhodamine 110, which exhibits excitation and emission wavelengths of 485nm and 525nm, respectively[1, 2]. (Z-Ala-Ala-Ala-Ala)2Rh110 demonstrates high specificity for elastase with minimal cross-reactivity, making it suitable for the precise quantitative analysis of enzymatic activity within complex biological samples. For instance, (Z-Ala-Ala-Ala-Ala)2Rh110 can be utilized to determine the enzymatic activity of neutrophil elastase (NE) in the bronchoalveolar lavage fluid (BALF) of mice infected with the influenza A virus (IAV)[3, 4].
References:
[1] Johnson A F, Struthers M D, Pierson K B, et al. Nonisotopic DNA detection system employing elastase and a fluorogenic rhodamine substrate[J]. Analytical chemistry, 1993, 65(17): 2352-2359.
[2] Khatib-Massalha E, Michelis R, Trabelcy B, et al. Free circulating active elastase contributes to chronic inflammation in patients on hemodialysis[J]. American Journal of Physiology-Renal Physiology, 2018, 314(2): F203-F209.
[3] Sule G, Abuaita B H, Steffes P A, et al. Endoplasmic reticulum stress sensor IRE1α propels neutrophil hyperactivity in lupus[J]. The Journal of clinical investigation, 2021, 131(7).
[4] Almutairi F, Sarr D, Tucker S L, et al. RGS10 reduces lethal influenza infection and associated lung inflammation in mice[J]. Frontiers in Immunology, 2021, 12: 772288.
(Z-Ala-Ala-Ala-Ala)2Rh110是一种灵敏的荧光弹性蛋白酶底物，无色且不发荧光的 (Z-Ala-Ala-Ala-Ala)2Rh110被弹性蛋白酶选择性裂解，产生强荧光化合物罗丹明110，罗丹明110的激发/发射波长为485nm/525nm[1, 2]。(Z-Ala-Ala-Ala-Ala)2Rh110对弹性蛋白酶具有高度特异性，交叉反应性低，能够用于复杂生物样本中酶活性的精准定量分析，例如(Z-Ala-Ala-Ala-Ala)2Rh110能够用于测定流感病毒（IAV）感染小鼠支气管肺泡灌洗液（BALFs）中的中性粒细胞弹性蛋白酶（NE）酶活性[3, 4]。