Vitamin B12是一种含钴的水溶性维生素，作为甲硫氨酸合酶和L-甲基丙二酰辅酶A变位酶的辅助因子。
Cas No.:68-19-9
Sample solution is provided at 25 µL, 10mM.
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Vitamin B12 is a water-soluble vitamin containing cobalt, serving as a cofactor for methionine synthase and L-methylmalonyl-CoA mutase. Vitamin B12 can participate in methyl transfer reactions to promote erythrocyte development and maturation. Vitamin B12 helps maintain the integrity of the myelin sheath to reduce neurological damage. Vitamin B12 can be used in research related to megaloblastic anemia and neurological disorders[1-4].
In vitro, SH-SY5Y neuroblastoma cells were pretreated with Vitamin B12 (10nM) for 12h, followed by stimulation with H₂O₂ (50μM) for 24h. Vitamin B12 significantly increased phospholipid levels and protected plasmalogens from oxidative stress, while enhancing the expression of antioxidant enzymes[5]. HeLa, HL-60, and MCF-7 cancer cells were treated with Vitamin B12 (0.01–1000nM) in combination with 1,25(OH)₂D₃ for 24–72 hours. Vitamin B12 significantly enhanced the cytotoxicity of 1,25(OH)₂D₃ and increased the activation of caspase 4 and 8[6].
In vivo, Elmo1H/H Ins2Akita/+ diabetic mice were supplemented with Vitamin B12 (10mg/kg/day) via drinking water for 8 weeks. Vitamin B12 significantly reduced glomerular mesangial expansion and altered the expression of circadian clock genes[7]. In a myocardial ischemia/reperfusion injury model, C57BL/6 mice were administered Vitamin B12 (50mg/L; 4mL/day) via drinking water for 7 consecutive days prior to surgery. Vitamin B12 significantly alleviated myocardial ischemia/reperfusion injury[8].
References:
[1] Romain M, Sviri S, Linton DM, et al. The role of Vitamin B12 in the critically ill--a review. Anaesth Intensive Care. 2016 Jul;44(4):447-52.
[2] ZUCKNER J. Vitamin B12 therapy in osteoarthritis. Mo Med. 1954 Jun;51(6):450-1.
[3] Hasbaoui BE, Mebrouk N, Saghir S, et al. Vitamin B12 deficiency: case report and review of literature. Pan Afr Med J. 2021 Mar 4;38:237.
[4] Langan RC, Goodbred AJ. Vitamin B12 Deficiency: Recognition and Management. Am Fam Physician. 2017 Sep 15;96(6):384-389.
[5] Theiss EL, Griebsch LV, Lauer AA, et al. Vitamin B12 Attenuates Changes in Phospholipid Levels Related to Oxidative Stress in SH-SY5Y Cells. Cells. 2022 Aug 18;11(16):2574.
[6] Atoum MF, Alzoughool FE, Al-Mazaydeh ZA, et al. Tahtamouni LH. Vitamin B12 enhances the antitumor activity of 1,25-dihydroxyvitamin D3 via activation of caspases and targeting actin cytoskeleton. Tumour Biol. 2022;44(1):17-35.
[7] Wariyapperuma Appuhamillage NMW, Deshmukh AA, Moser RL, et al. Vitamin B12 Protects Against Early Diabetic Kidney Injury and Alters Clock Gene Expression in Mice. Biomolecules. 2025 Dec 3;15(12):1689.
[8] Qin Y, Shi Y, Yu Q, et al. Vitamin B12 alleviates myocardial ischemia/reperfusion injury via the SIRT3/AMPK signaling pathway. Biomed Pharmacother. 2023 Jul;163:114761.
Vitamin B12是一种含钴的水溶性维生素，作为甲硫氨酸合酶和L-甲基丙二酰辅酶A变位酶的辅助因子。Vitamin B12可通过参与甲基转移反应以促进红细胞发育与成熟，同时通过维持神经髓鞘完整性以减少神经系统损伤。Vitamin B12可用于巨幼细胞性贫血和神经系统病变的相关研究[1-4]。
在体外，Vitamin B12（10nM）预处理SH-SY5Y神经母细胞瘤细胞12h，随后以H₂O₂（50μM）刺激24h。Vitamin B12显著提高磷脂水平并保护plasmalogens免受氧化应激，同时提高抗氧化酶表达[5]。Vitamin B12（0.01-1000nM）与1,25(OH)2D3联合处理HeLa、HL-60、MCF-7等癌细胞24-72小时。Vitamin B12显著增强1,25(OH)2D3的细胞毒性，同时增加caspase 4和8的激活[6]。
在体内，Vitamin B12（10mg/kg/day）通过饮用水给药，用于处理Elmo1H/H Ins2Akita/+糖尿病小鼠8周。Vitamin B12显著减少了肾小球系膜扩张，同时改变了生物钟基因表达[7]。在缺血/再灌注损伤术前连续7天，Vitamin B12（50mg/L；4mL/day）通过饮用水给药于C57BL/6小鼠。Vitamin B12显著减轻了心肌缺血/再灌注损伤[8]。
| Cell experiment [1]: | |
Cell lines | SH-SY5Y neuroblastoma cells |
Preparation Method | SH-SY5Y cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C and 5% CO₂. When 80% confluence was reached, the FBS content was reduced to 1% for 16h, followed by incubation with 10nM Vitamin B12 for 24h. |
Reaction Conditions | Vitamin B12 (10nM); 12h; followed by stimulation with H₂O₂ (50μM); 24h |
Applications | Vitamin B12 treatment resulted in elevated levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), and plasmalogens. Vitamin B12 protected plasmalogens from hydrogen peroxide (H₂O₂)-induced oxidative stress due to an elevated expression of the ROS-degrading enzymes superoxide dismutase (SOD) and catalase (CAT). Furthermore, Vitamin B12 elevates plasmalogen synthesis by increasing the expression of alkylglycerone phosphate synthase (AGPS) and choline phosphotransferase 1 (CHPT1) in SH-SY5Y cells exposed to H₂O₂-induced oxidative stress. |
| Animal experiment [2]: | |
Animal models | Elmo1H/H Ins2Akita/+ diabetic mice (C57BL/6J genetic background) |
Preparation Method | Mice were supplemented with Vitamin B12 (10mg/kg/day) via drinking water starting at eight weeks of age for eight weeks. |
Dosage form | 10mg/kg/day; drinking water; daily for 8 weeks |
Applications | Vitamin B12 treatment significantly reduced the percentage of glomeruli with mesangial expansion, improved systolic blood pressure and plasma triglyceride levels, and altered the expression of circadian rhythm genes in diabetic kidneys. |
References: | |
| Cas No. | 68-19-9 | SDF | |
| 别名 | 维生素B12; Cyanocobalamin | ||
| Canonical SMILES | CC1=CC2=C(C=C1C)N(C=N2)C3C(C(C(O3)CO)OP(=O)([O-])OC(C)CNC(=O)CCC4(C(C5C6(C(C(C(=C(C7=NC(=CC8=NC(=C(C4=N5)C)C(C8(C)C)CCC(=O)N)C(C7(C)CC(=O)N)CCC(=O)N)C)[N-]6)CCC(=O)N)(C)CC(=O)N)C)CC(=O)N)C)O.[C-]#N.[Co+3] | ||
| 分子式 | C63H88CoN14O14P | 分子量 | 1355.37 |
| 溶解度 | ≥ 45.65mg/mL in DMSO | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 737.8 μL | 3.689 mL | 7.3781 mL |
| 5 mM | 147.6 μL | 737.8 μL | 1.4756 mL |
| 10 mM | 73.8 μL | 368.9 μL | 737.8 μL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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- Purity: >98.00% Appearance: A solid
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