trans-Zeatin is a natural cytokinin, a plant growth regulator that plays a central role in plant cell growth, differentiation, and division[1, 2]. trans-Zeatin can be used as a culture medium additive to induce plant callus growth and bud formation[3]. trans-Zeatin is a potential therapeutic agent for skin photoaging[4].
In vitro, treatment of human skin fibroblasts (HSFs) with trans-Zeatin (20-80μM) for 24h enhanced the cell viability of UVB-irradiated HSFs and significantly inhibited UVB-induced matrix metalloproteinase-1 (MMP-1) expression[5]. Treatment of skin keratinocytes (HaCaT cells) with trans-Zeatin (20-80μM) for 4-24h upregulated the expression of aquaporin-3 (AQP3) in HaCaT cells in a time- and dose-dependent manner[6].
References:
[1] Schäfer M, Brütting C, Meza-Canales I D, et al. The role of cis-zeatin-type cytokinins in plant growth regulation and mediating responses to environmental interactions[J]. Journal of experimental botany, 2015, 66(16): 4873-4884.
[2] Großkinsky D, Edelsbrunner K, Pfeifhofer H, et al. Cis-and trans-zeatin differentially modulate plant immunity[J]. Plant signaling & behavior, 2013, 8(7): e24798.
[3] Cardoza V. Tissue culture: The manipulation of plant development[J]. Plant Biotechnology and Genetics: principles, techniques, and applications, 2008: 113-134.
[4] Kesharwani A, Panda S P. Isolation of Phytohormone trans-Zeatin: Potential Oxidant Scavenger and Anti-Aging Compound[J]. Neurochemical Journal, 2024, 18(1): 134-146.
[5] Yang B, Ji C, Kang J, et al. Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts[J]. International Journal of Molecular Medicine, 2009, 23(4): 555-560.
[6] Ji C, Yang Y, Yang B, et al. Trans-Zeatin attenuates ultraviolet induced down-regulation of aquaporin-3 in cultured human skin keratinocytes[J]. International journal of molecular medicine, 2010, 26(2): 257-263.
trans-Zeatin是一种天然的细胞分裂素（cytokinin），属于植物生长调节剂，在植物细胞生长、分化和分裂中起核心作用[1, 2]。trans-Zeatin能够用作培养基的添加剂，用于诱导植物愈伤组织生长和芽的形成[3]。trans-Zeatin是一种潜在的皮肤光老化治疗药物[4]。
在体外，trans-Zeatin（20-80μM）处理人皮肤成纤维细胞（HSFs）24h，增强了接受UVB照射的HSFs的细胞活力，显著抑制了UVB诱导的基质金属蛋白酶-1（MMP-1）表达[5]。trans-Zeatin（20-80μM）处理皮肤角质形成细胞（HaCaT 细胞）4-24h，以时间和剂量依赖性的方式上调了HaCaT细胞中的水通道蛋白-3（AQP3）的表达[6]。