TMRE (Tetramethylrhodamine ethyl ester perchlorate) a highly fluorescent, cationic, lipophilic dye, and displays excitation/emission spectra of 561/582nm [1]. The retention of TMRE depends on the mitochondrial transmembrane potential, which has been used for imaging individual mitochondria in living cells or for separating living cells from non-apoptotic cells [2]. TMRE has been widely used to assess the changes in mitochondrial membrane potential induced by radiation, and to study the damage caused by radiation to cells[3].
References:
[1] Barteneva N S, Ponomarev E D, Tsytsykova A, et al. Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting[J]. Journal of Histochemistry & Cytochemistry, 2014, 62(4): 265-275.
[2] Jayaraman S. Flow cytometric determination of mitochondrial membrane potential changes during apoptosis of T lymphocytic and pancreatic beta cell lines: comparison of tetramethylrhodamineethylester (TMRE), chloromethyl-X-rosamine (H2-CMX-Ros) and MitoTracker Red 580 (MTR580)[J]. Journal of immunological methods, 2005, 306(1-2): 68-79.
[3] Walsh D W M, Siebenwirth C, Greubel C, et al. Live cell imaging of mitochondria following targeted irradiation in situ reveals rapid and highly localized loss of membrane potential[J]. Scientific Reports, 2017, 7(1): 46684.
TMRE (Tetramethylrhodamine ethyl ester perchlorate)是一种高荧光、阳离子、亲脂性染料，激发/发射光谱为561/582nm[1]。TMRE的滞留依赖于线粒体跨膜电位，该特性已被用于对活细胞中的单个线粒体进行成像，或用于从非凋亡细胞中分离活细胞[2]。TMRE已被广泛用于评估辐射诱导的线粒体膜电位变化，并研究辐射对细胞造成的损伤[3]。