Tiopinac (RS 40974)

Animal experiment:

Female rats, 160-180 g, receive 0.1 mL of a suspension in mineral oil of heatkilled M. butyricum (10 mg/mL) by means of two intradermal injections (0.05 mL each) into the proximal 1/4 of the tail on day 0. When the test agent is studied for its activity versus the development of the arthritic syndrome, it is administered p.o. (0.5 mL vehicle/dose) twice each day (at 9 a.m. and 4 p.m.) for 17 days, beginning on day 1. On day 18 the intensity of the swelling of the four foot pads and tail is determined blindly utilizing a scoring system in which the swelling in the four paws is scored 0-4 for each paw and the tail swelling is scored 0-3. The total maximum score is 19. The body weights and the weights of both hind paws are obtained. This procedure is a modification of a system initially described by PEARSON.

References:

[1]. Rooks WH 2nd, et al. The anti-inflammatory and analgesic profile of 6,11-dihydrodibenzo-[b.e.]-thiepin-11-one-3-acetic acid (tiopinac). Agents Actions. 1980 Jun;10(3):266-73.