Thiazovivin is a selective Rho-associated kinase (ROCK) inhibitor [1]. Thiazovivin selectively inhibits the ROCK signaling pathway, reduces cytoskeletal tension, reduces stress-induced cell apoptosis, promotes cell adhesion and spreading, and thus improves cell survival in a single-cell state [2-3]. Thiazovivin is often used in stem cell research and cell engineering [4].
In human corneal endothelial cells (HCEC), Thiazovivin (2µM, 4µM, 6µM; 24h, 48h) improve HCEC morphology, cell junctions, and ion transporter expression by inhibiting EndMT/EMT [5]. In human embryonic stem cells, Thiazovivin (2µM; 24h) treatment improves the maintenance of stemness in a putative stem-like cell populations of cattle by promoting the expression of pluripotency marker genes, as well as enhancing the expression of the E-cadherin gene, resulting in an increase in cell adhesion [1]. In human corneal endothelial cells, Thiazovivin (2µM, 4µM, 6µM; 24h) treatment improved cell morphology, connectivity and function, and significantly inhibited the expression of ROCK protein [6]. In bovine embryo, Thiazovivin (2µM; 96h) treatment improves embryo survival and hatching rates [7]. In human smooth muscle cells, Thiazovivin (2µM; 2h) reverses AngII-induced phosphorylation of MYPT1 and MLC [8].
References:
[1]. Park S, Kim D, Jung YG, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Animal reproduction science. 2015 Oct 1; 161: 47-57.
[2]. Mohseni R, Shoae‐Hassani A, Verdi J. Reprogramming of endometrial adult stromal cells in the presence of a ROCK inhibitor, thiazovivin, could obtain more efficient iPSCs. Cell biology international. 2015 May; 39(5): 515-518.
[3]. Laowtammathron C, Chingsuwanrote P, Srisook P, et al. The novel combination of small-molecule inhibitors increases the survival and colony formation capacity of human induced pluripotent stem cells after single-cell dissociation. Science Progress. 2025 Apr; 108(2): 00368504251330956.
[4]. Rizzino A. Stimulating progress in regenerative medicine: improving the cloning and recovery of cryopreserved human pluripotent stem cells with ROCK inhibitors. Regenerative medicine. 2010 Sep 1; 5(5): 799-807.
[5]. Wu Q, Ouyang C, Xie L, et al. The ROCK inhibitor, thiazovivin, inhibits human corneal endothelial‑to‑mesenchymal transition/epithelial‑to‑mesenchymal transition and increases ionic transporter expression. International journal of molecular medicine. 2017 Oct 1; 40(4): 1009-1018.
[6]. Wu QN, Ling YZ, Ouyang C, et al. Effect of a new ROCK inhibitor thiazovivin on the morphology and function of human corneal endothelial cells. [Zhonghua yan ke za Zhi] Chinese Journal of Ophthalmology. 2016 Sep 1; 52(9): 686-692.
[7]. Kim D, Sul H, Jung YG, et al. Holding of bovine blastocysts at suprazero temperatures using small molecules. Scientific Reports. 2017 Aug 25; 7(1): 9490.
[8]. Cao X, Luo T, Luo X, et al. Resveratrol prevents AngII-induced hypertension via AMPK activation and RhoA/ROCK suppression in mice. Hypertension Research. 2014 Sep; 37(9): 803-810.
Thiazovivin是一种选择性Rho相关激酶（ROCK）抑制剂 [1]。Thiazovivin选择性抑制ROCK信号通路，降低细胞骨架张力，减少应激诱导的细胞凋亡，促进细胞粘附和扩散，从而提高单细胞状态下的细胞存活率 [2-3]。Thiazovivin常用于干细胞研究和细胞工程 [4]。
在人角膜内皮细胞（HCEC）中，Thiazovivin（2µM、4µM、6µM；24h、48h）通过抑制EndMT/EMT来改善HCEC的形态、细胞连接和离子转运蛋白的表达 [5]。在人类胚胎干细胞中，Thiazovivin（2µM；24h）处理可促进多能性标记基因的表达，并增强E-钙粘蛋白基因的表达，从而提高牛假定类干细胞群的干性维持，从而增加细胞粘附性 [1]。在人类角膜内皮细胞中，Thiazovivin（2µM、4µM、6µM；24h）处理可改善细胞形态、连通性和功能，并显著抑制ROCK蛋白的表达 [6]。在牛胚胎中，Thiazovivin（2µM；96h）处理可提高胚胎存活率和孵化率 [7]。在人类平滑肌细胞中，Thiazovivin（2µM；2h）可逆转血管紧张素Ⅱ诱导的MYPT1和MLC磷酸化 [8]。