Temozolomide acid is an active metabolite of the alkylating agent precursor temozolomide .1 It is cytotoxic to TLX5 murine lymphoma cells in a concentration-dependent manner. Temozolomide acid is also a synthetic intermediate in the synthesis of temozolomide prodrugs with anticancer activity.2
1.Tsang, L.L.H., Farmer, P.B., Gescher, A., et al.Characterisation of urinary metabolites of temozolomide in humans and mice and evaluation of their cytotoxicityCancer Chemother. Pharmacol.26(6)429-436(1990) 2.Liu, D., Yang, J.-G., Cheng, J., et al.Synthesis and antitumor activity of 3-methyl-4-oxo-3,4-dihydroimidazo [5,1-d][1,2,3,5]tetrazine-8-carboxylates and -carboxamidesMolecules15(12)9427-9437(2010)