GlpBio

T4 DNA

目录号: GP22276纯度: >98%
COASDSData Sheet
T4 DNA Ligase Recombinant
T4 DNA
规格价格库存数量操作
20,000IU¥910.00现货
1
100,000IU¥3,570.00现货
1
500,000IU¥13,860.00现货
1
电话: 400-920-5774Email: sales@glpbio.cn

文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例
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    Nature
    641, 529–536 (2025)
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    Nature
    628, 630–638 (2024)
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    Nature
    632, 686–694 (2024)
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    Nature
    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
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    Science
    387(6739) (2025)
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    Science
    387(6734) (2025)
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    Cell Research
    35, 97–116 (2025)
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    Cell Research
    34, 683–706 (2024)
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    Cell Research
    33, 273–287 (2023)
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    33, 546–561 (2023)
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    33, 904–922 (2023)
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    Cell Research
    31, 1291–1307 (2021)

产品描述 Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

产品文档 Product Documents

Purity:>98%
COASDSData Sheet

Product Data

Source
Escherichia Colilambda lysogen NM 989.
Physical Appearance
Sterile filtered liquid formulation having a concentration of 167,000 U/ml.
Shipping Condition
Shipped with Ice Packs.
Synonyms
DNA ligase 4; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4.
Biological Activity
One Weiss unit is equivalent to circa 67 cohesive-end ligation units.? T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.? Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50?M.? To dilute T4 DNA Ligase that will subsequently be stored at иC20°C , 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.
Formulation
50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 ?m in 5? termini). Optimal ligation occurs at 16C.