Sulfosuccinimidyl oleate (SSO) is an irreversible inhibitor of the fatty acid translocase CD36, blocking uptake of oleate, linoleate, or stearate by about 65% when added at 200 ?M to adipocytes.1,2 It reduces the uptake of palmitate by mouse insulinoma MIN6 cells, preventing palmitate-
1.Harmon, C.M., Luce, P., Beth, A.H., et al.Labeling of adipocyte membranes by sulfo-N-succinimidyl derivatives of long-chain fatty acids: Inhibition of fatty acid transportJ. Membr. Biol.121(3)261-268(1991) 2.Abumrad, N.A., el-Maghrabi, M.R., Amri, E.Z., et al.Cloning of a rat adipocyte membrane protein implicated in binding or transport of long-chain fatty acids that is induced during preadipocyte differentiation. Homology with human CD36J. Biol. Chem.268(24)17665-17668(1993) 3.Noushmehr, H., D'Amico, E., Farilla, L., et al.Fatty acid translocase (FAT/CD36) is localized on insulin-containing granules in human pancreatic β-cells and mediates fatty acid effects on insulin secretionDiabetes54(2)472-481(2005) 4.Nicholls, H.T., Kowalski, G., Kennedy, D.J., et al.Hematopoietic cell-restricted deletion of CD36 reduces high-fat diet-induced macrophage infiltration and improves insulin signaling in adipose tissueDiabetes60(4)1100-1110(2011)