Pyrroloquinoline quinone

Pyrroloquinoline quinone was reported to be a new mammalian lactate dehydrogenase (LDH) coenzyme ^[1]. Pyrroloquinoline quinone has diverse effects including an antioxidant effect, a cell growth-promoting effect, and a stimulatory effect on mitochondriogensis ^{[2,3,4,5,6,7]}

Pyrroloquinoline quinone protected against oxidative damage caused by H₂O₂, because it has many reducing sites. It was suggested that HepG2 cells require more pyrroloquinoline quinone due to the greater damage caused by hydrogen peroxide than do SK-N-SH cells ^[8]. Pyrroloquinoline quinone showed protect effect on 6-OHDA using human neuroblastoma SH-SY5Y cells at 15 μM ^[9].

Pyrroloquinoline quinone can serve as a growth factor in BALB/c mice [10]. The lower values for RQ in PQQ-deficient mice compared with PQQ-supplemented mice, as a sign of compromised mitochondrial function and inefficient carbohydrate utilization ^[11].

References:
[1]. Akagawa M, Minematsu K, Shibata T, et al. Identification of lactate dehydrogenase as a mammalian pyrroloquinoline quinone (PQQ)-binding protein[J]. Scientific reports, 2016, 6(1): 1-19.
[2]. He K, Nukada H, Urakami T, et al. Antioxidant and pro-oxidant properties of pyrroloquinoline quinone (PQQ): implications for its function in biological systems[J]. Biochemical pharmacology, 2003, 65(1): 67-74.
[3]. Nunome K, Miyazaki S, Nakano M, et al. Pyrroloquinoline quinone prevents oxidative stress-induced neuronal death probably through changes in oxidative status of DJ-1[J]. Biological and Pharmaceutical Bulletin, 2008, 31(7): 1321-1326.
[4]. Zhang Y, Feustel P J, Kimelberg H K. Neuroprotection by pyrroloquinoline quinone (PQQ) in reversible middle cerebral artery occlusion in the adult rat[J]. Brain research, 2006, 1094(1): 200-206.
[5]. Yamaguchi K, Sasano A, Urakami T, et al. Stimulation of nerve growth factor production by pyrroloquinoline quinone and its derivatives in vitro and in vivo[J]. Bioscience, biotechnology, and biochemistry, 1993, 57(7): 1231-1233.
[6]. Stites T, Storms D, Bauerly K, et al. Pyrroloquinoline quinone modulates mitochondrial quantity and function in mice[J]. The Journal of nutrition, 2006, 136(2): 390-396.
[7]. Chowanadisai W, Bauerly K A, Tchaparian E, et al. Pyrroloquinoline quinone stimulates mitochondrial biogenesis through cAMP response element-binding protein phosphorylation and increased PGC-1α expression[J]. Journal of Biological Chemistry, 2010, 285(1): 142-152.
[8]. Yamada Y, Nishii K, Kuwata K, et al. Effects of pyrroloquinoline quinone and imidazole pyrroloquinoline on biological activities and neural functions[J]. Heliyon, 2020, 6(1): e03240.
[9]. Hara H, Hiramatsu H, Adachi T. Pyrroloquinoline quinone is a potent neuroprotective nutrient against 6-hydroxydopamine-induced neurotoxicity[J]. Neurochemical research, 2007, 32(3): 489-495.
[10]. Naito Y, Kumazawa T, Kino I, et al. Effects of pyrroloquinoline quinone (PQQ) and PQQ-oxazole on DNA synthesis of cultured human fibroblasts[J]. Life sciences, 1993, 52(24): 1909-1915.
[11]. Stites T, Storms D, Bauerly K, et al. Pyrroloquinoline quinone modulates mitochondrial quantity and function in mice[J]. The Journal of nutrition, 2006, 136(2): 390-396.

据报道,吡咯并喹啉醌是一种新的哺乳动物乳酸脱氢酶 (LDH) 辅酶^[1]。吡咯并喹啉醌具有多种作用,包括抗氧化作用、细胞生长促进作用和对线粒体生成的刺激作用^{[2,3,4,5,6,7]}

吡咯并喹啉醌可防止 H₂O₂ 引起的氧化损伤,因为它具有许多还原位点。提示 HepG2 细胞由于过氧化氢引起的损伤比 SK-N-SH 细胞需要更多的吡咯并喹啉醌^[8]。使用人神经母细胞瘤 SH-SY5Y 细胞,15 μM 吡咯并喹啉醌对 6-OHDA 具有保护作用 ^[9]。

吡咯并喹啉醌可作为 BALB/c 小鼠的生长因子 [10]。与补充 PQQ 的小鼠相比,PQQ 缺陷小鼠的 RQ 值较低,表明线粒体功能受损和碳水化合物利用效率低下^[11]。