Pumaprazole (BY-841)

Animal experiment:

The pylorus of female Sprague-Dawley rats (160 to 220 g body weight) is ligated under ether anesthesia, the abdomen is closed and 100 mg/kg of acetylsalicylic acid (ASA) in 10 mL/kg are given orally. Following ASA administration, Pumaprazole (solution) or vehicle (i.v. saline or tap water in case of oral administration) is given i.v. in 1 mL/kg or i.d. in 2.5 mL/kg. Oral drug administration (in 10 mL/kg) is 1 h before pylorus ligation. Four hours after pylorus ligation, the stomach is excised, carefully keeping the esophagus closed, opened along the greater curvature and the luminal contents are removed, centrifuged, the volume is measured and the acidity determined by titration with 0.1 N NaOH to pH 7[1].

References:

[1]. Kromer W, et al. Animal pharmacology of reversible antagonism of the gastric acid pump, compared to standard antisecretory principles. Pharmacology. 2000 May;60(4):179-87.