Pregnanediol is the main metabolite of progesterone and is excreted in the urine [1]. The Pregnanediol excreted by pregnant women can reflect the function of the placenta, and urine progesterone can be used to assess the function of the ovaries [2]. Pregnanediol can be used as a reference substance to develop new detection methods for improving the identification of related compounds[3]. When zebrafish embryos were exposed to waste water containing 300ng/l Pregnanediol for 48 hours, the spontaneous muscle contraction frequency significantly decreased, and the heart rate of the embryos significantly reduced[4].
References:
[1] Usuki S, Kondoh K, Kubo T. Plasma endothelin and LH-RH, LH, FSH, prolactin, progesterone, 17α-hydroxyprogesterone, estrone, 17β-estradiol, Δ4-androstenedione, testosterone, active renin, angiotensin-II and ANP levels in blood and LH, estrone and 17β-estradiol and pregnanediol levels in urine of normal cycling women[J]. Journal of Cardiovascular Pharmacology, 2000, 36: S421-S427.
[2] Haran S, Chindera K, Sabry M, et al. Natural Killer Cell Dysfunction in Premenopausal BRCA1 Mutation Carriers: A Potential Mechanism for Ovarian Carcinogenesis[J]. Cancers, 2024, 16(6): 1186.
[3] Zhang K, Fent K. Determination of two progestin metabolites (17α-hydroxypregnanolone and pregnanediol) and different classes of steroids (androgens, estrogens, corticosteroids, progestins) in rivers and wastewaters by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)[J]. Science of the Total Environment, 2018, 610: 1164-1172.
[4] Zhang K, Zhao Y, Fent K. Occurrence and ecotoxicological effects of free, conjugated, and halogenated steroids including 17α-hydroxypregnanolone and pregnanediol in Swiss wastewater and surface water[J]. Environmental science & technology, 2017, 51(11): 6498-6506.
Pregnanediol是孕酮的主要代谢产物，通过尿液排出体外[1]。孕妇排泄的Pregnanediol可反映胎盘功能，而尿液中Pregnanediol的检测可用于评估卵巢功能[2]。Pregnanediol可作为参照物，用于开发新的检测方法以提升相关化合物的鉴定能力[3]。当斑马鱼胚胎在含有300ng/l Pregnanediol的废水中暴露48小时后，自发性肌肉收缩频率显著降低，同时胚胎心率明显减慢[4]。