Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5? to 3? direction in the existence of magnesium. Pfu DNA Polymerase possesses 3? to 5? exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
Pfu DNA Polymerase
目录号: GP22070纯度: >98%
Pfu-DNA Polymerase Recombinant
| 规格 | 价格 | 库存 | 数量 | 操作 |
|---|---|---|---|---|
| 500U | ¥3,570.00 | 现货 | 1 | |
| 2500U | ¥14,000.00 | 现货 | 1 | |
| 100U | ¥2,030.00 | 现货 | 1 |
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文献被引
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Nature641, 529–536 (2025)
Nature628, 630–638 (2024)
Nature632, 686–694 (2024)
Nature618, 1017–1023 (2023)
Nature610, 366–372 (2022)
Cell187(9):2288-2304 (2024)
Cell183(7):1867-1883 (2020)
Science388(6745) (2025)
Science387(6739) (2025)
Science387(6734) (2025)
Cell Research35, 97–116 (2025)
Cell Research34, 683–706 (2024)
Cell Research33, 273–287 (2023)
Cell Research33, 546–561 (2023)
Cell Research33, 904–922 (2023)
Cell Research31, 1291–1307 (2021)
产品描述 Description
产品文档 Product Documents
Purity:>98%
Product Data
Source
Escherichia Coli.
Physical Appearance
Sterile liquid formulation.
Shipping Condition
Shipped with Ice Packs.
Synonyms
DNA polymerase; EC 2.7.7.7; Pfu polymerase; Pfu-DNA Polymerase.
Stability
Pfu DNA Polymerase although stable at 10°C for 5 days, should be stored below -18°C .Please prevent freeze-thaw cycles.
Formulation
50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol.