p-Aminophenylmercuric acetate是一种有机汞化合物，可作为MMP激活剂，能够作用于蛋白质巯基或诱导与MMP-8激活和抑制均相关的半胱氨酸转换反应。
Cas No.:6283-24-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
p-Aminophenylmercuric acetate, an organomercurial MMP activator capable of attacking protein sulfhydryl groups or inducing cysteine switch reactions involved in both the activation and inhibition of MMP-8 [1]. p-Aminophenylmercuric acetate can convert the latent form of MMP-9 (94kDa) purified from the human cell line HL-60 into an intermediary active form (84kDa)[2]. p-Aminophenylmercuric acetate can be used as a building block to synthesize novel types of photochromic dyes[3].
In vitro, p-Aminophenylmercuric acetate (30µM; 20min) reduced the number of dihydromorphane binding sites in rat brain homogenate and increased the sensitivity of the agonist to the inhibitory effect on sodium binding[4]. p-Aminophenylmercuric acetate treatment (100µM) for 1h activated the betacellulin precursor (pro-BTC) shedding in Wild-type dermal fibroblasts transfected with BTC-hemagglutinin (HA)[5]. Treatment with p-Aminophenylmercuric acetate (>0.3μg/ml) for 24 hours significantly induced the death of murine lymphocytes[6].
References:
[1] Gendron R, Grenier D, Sorsa T, et al. Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine[J]. Clinical Diagnostic Laboratory Immunology, 1999, 6(3): 437-439.
[2] Backstrom J R, Tökés Z A. The 84‐kDa form of human matrix metalloproteinase‐9 degrades substance P and gelatin[J]. Journal of neurochemistry, 1995, 64(3): 1312-1318.
[3] Kanakkanatt S V, Patel V S, Patel R G, et al. Novel Methods of Synthesis of Dithizonate Type Photochromic Dyes & Polymers[J]. Molecular Crystals and Liquid Crystals Science and Technology. Section A. Molecular Crystals and Liquid Crystals, 1994, 246(1): 159-168.
[4] PASTERNAK G W, WILSON H A, SNYDER S H. Differential effects of protein-modifying reagents on receptor binding of opiate agonists and antagonists[J]. Molecular Pharmacology, 1975, 11(3): 340-351.
[5] Sanderson M P, Erickson S N, Gough P J, et al. ADAM10 mediates ectodomain shedding of the betacellulin precursor activated by p-aminophenylmercuric acetate and extracellular calcium influx[J]. Journal of Biological Chemistry, 2005, 280(3): 1826-1837.
[6] Kim H M, Hong D H, Lee C W, et al. Studies on cell-specific cytotoxicities induced by p-aminophenyl-mercuric acetate (APMA)[J]. The Journal of Toxicological Sciences, 1998, 23(SupplementIV): 653-656.
p-Aminophenylmercuric acetate是一种有机汞化合物，可作为MMP激活剂，能够作用于蛋白质巯基或诱导与MMP-8激活和抑制均相关的半胱氨酸转换反应[1]。p-Aminophenylmercuric acetate能将从人源细胞系HL-60中纯化的无活性前体形式MMP-9（94kDa）转化为中间活性形式（84kDa）[2]。p-Aminophenylmercuric acetate可作为结构单元用于合成新型光致变色染料[3]。
在体外，p-Aminophenylmercuric acetate处理（30µM；20分钟）减少了大鼠脑匀浆中二氢吗啡结合位点的数量，并提高了激动剂对钠结合抑制效应的敏感性[4]。p-Aminophenylmercuric acetate处理（100µM）1小时激活了转染BTC-hemagglutinin (HA)的野生型皮肤成纤维细胞中betacellulin前体（pro-BTC）的脱落过程[5]。使用浓度高于0.3µg/ml的p-Aminophenylmercuric acetate处理24小时，可显著诱导小鼠淋巴细胞死亡[6]。
| Cell experiment [1]: | |
Cell lines | Wild-type dermal fibroblasts transfected with BTC-HA |
Preparation Method | Wild-type dermal fibroblasts transfected with BTC-HA were seeded at a density of 1×105 cells per well in a 6-well plate. Cells were cultured in a medium containing IFN-γ at 33°C for 48 hours until confluence was reached, and then further cultured in a medium without IFN-γ at 37°C for 36 hours. To evaluate the stimulation-induced pro-BTC shedding, the cells were cultured in serum-free DMEM medium containing or without p-Aminophenylmercuric acetate (100μM) for 1h. 30 minutes before the addition of the p-Aminophenylmercuric acetate, the cells were pre-incubated with GM6001, kinase inhibitors, or calcium-free DMEM. Then, the cell lysates were collected for analysis. |
Reaction Conditions | 100µM; 1h |
Applications | p-Aminophenylmercuric acetate treatment activated the Pro-BTC shedding in wild-type dermal fibroblasts transfected with BTC-HA. |
References: | |
| Cas No. | 6283-24-5 | SDF | Download SDF |
| 别名 | APMA | ||
| 分子式 | C8H9HgNO2 | 分子量 | 351.75 |
| 溶解度 | DMSO : 125 mg/mL (355.37 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.8429 mL | 14.2146 mL | 28.4293 mL |
| 5 mM | 568.6 μL | 2.8429 mL | 5.6859 mL |
| 10 mM | 284.3 μL | 1.4215 mL | 2.8429 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet