OUL35 is an inhibitor of poly(ADP-ribose) polymerase 10 (PARP10; IC50 = 329 nM).1 It is selective for PARP10 over a panel of 15 PARP enzymes, including PARP4, PARP14, and PARP16 (IC50s = 22.6, 23.4, and 4.17 μM, respectively). OUL35 (10 μM) reverses inhibition of HeLa cell proliferation induced by PARP10 overexpression. It sensitizes HeLa cells to hydroxyurea , increasing hydroxyurea-induced inhibition of cell proliferation when used at a concentration of 5 μM.
|1. Venkannagari, H., Verheugd, P., Koivunen, J., et al. Small-molecule chemical probe rescues cells from mono-ADP-ribosyltransferase ARTD10/PARP10-induced apoptosis and sensitizes cancer cells to DNA damage. Cell Chem. Bio. 23(10), 1251-1260 (2016).