O-Propargyl-Puromycin (O-Propargylpuromycin) is a derivative synthesized by replacing the O-methyl-phenyl ring of puromycin with an alkyne group. O-Propargyl-Puromycin allows subsequent manipulation of puromycylated proteins by copper-catalyzed alkyne-azide cycloaddition of a ‘clickable’ biotin or fluorophore[1].
References:
[1] Aviner R. The science of puromycin: From studies of ribosome function to applications in biotechnology. Comput Struct Biotechnol J. 2020 Apr 24;18:1074-1083.
O-Propargyl-Puromycin (O-Propargylpuromycin)是通过将嘌呤霉素的O-甲基苯基环替换为炔基(alkyne)而合成的衍生物。O-Propargyl-Puromycin可通过铜催化的炔-叠氮环加成反应对嘌呤霉素化的蛋白质进行后续操作，例如连接“可点击”的生物素或荧光团[1]。