Neurotensin is a neuropeptide composed of 13 amino acids, which functions as a neurotransmitter, hormone, and neuromodulator. Neurotensin lowers blood pressure, inhibits gastrointestinal motility and gastric acid secretion to regulate digestive function, while also modulating ceramide synthesis via the NTS-NTSR2 signaling pathway to reduce inflammation and regulate metabolism. Neurotensin can be used in research related to neuropsychiatric disorders (such as schizophrenia and Parkinson's disease) and metabolic diseases (such as obesity and metabolic-associated fatty liver disease)[1-4].
In vitro, Neurotensin (1μM) treated MKN-1 and MKN-45 gastric cancer cells for 5 minutes. Neurotensin activated the expression and activity of matrix metalloproteinase-9 (MMP-9) through the NTR1 receptor, promoting cell invasion and migration[5]. Neurotensin (0.1μM) treated Calu-3, NCI-H358, and NCI-H441 non-small cell lung cancer cells for 5 minutes. Neurotensin significantly increased HER3 tyrosine phosphorylation and promoted the formation of EGFR/HER3 and HER2/HER3 heterodimers[6].
In vivo, Neurotensin (1pg–25ng; single injection) was administered intracerebroventricularly to CD-1 mice. Neurotensin significantly antagonized the analgesic effect induced by morphine[7]. Neurotensin (10μg; single injection) was administered intrathecally to C57BL/6 mice. Neurotensin significantly alleviated thermal pain and formaldehyde-induced pain[8].
References:
[1] Staley J, Fiskum G, Davis TP, et al. Neurotensin elevates cytosolic calcium in small cell lung cancer cells. Peptides. 1989 Nov-Dec;10(6):1217-21.
[2] Ehlers RA, Zhang Y, Hellmich MR, et al. Neurotensin-mediated activation of MAPK pathways and AP-1 binding in the human pancreatic cancer cell line, MIA PaCa-2. Biochem Biophys Res Commun. 2000 Mar 24;269(3):704-8.
[3] Okamoto A, Nakanishi T, Tonai S, et al. Neurotensin induces sustainable activation of the ErbB-ERK1/2 pathway, which is required for developmental competence of oocytes in mice. Reprod Med Biol. 2024 Mar 20;23(1):e12571.
[4] Banerjee M, Song J, Yan B, et al. Neurotensin promotes hepatic steatosis by regulating lipid uptake and mitochondrial adaptation in hepatocytes. Cell Death Dis. 2025 Apr 27;16(1):347.
[5] Akter H, Park M, Kwon OS, et al. Activation of matrix metalloproteinase-9 (MMP-9) by neurotensin promotes cell invasion and migration through ERK pathway in gastric cancer. Tumour Biol. 2015 Aug;36(8):6053-62.
[6] Moody TW, Ramos-Alvarez I, Jensen RT. Adding of neurotensin to non-small cell lung cancer cells increases tyrosine phosphorylation of HER3. Peptides. 2022 Oct;156:170858.
[7] Holmes BB, Rady JJ, Smith DJ, et al. Supraspinal neurotensin-induced antianalgesia in mice is mediated by spinal cholecystokinin. Jpn J Pharmacol. 1999 Feb;79(2):141-9.
[8] Barnwal A, Tamang R, Sanjeev Das, et al. Ponatinib delays the growth of solid tumours by remodelling immunosuppressive tumour microenvironment through the inhibition of induced PD-L1 expression. Br J Cancer. 2023 Oct;129(6):1007-1021.
Neurotensin是一种由13个氨基酸组成的神经肽，具有神经递质、激素和神经调质功能。Neurotensin可降低血压、抑制胃肠运动及胃酸分泌来调节消化功能，同时通过NTS-NTSR2信号通路调控神经酰胺合成以减少炎症和调节代谢。Neurotensin可用于神经精神疾病（如精神分裂症、帕金森病）和代谢疾病（如肥胖、代谢相关脂肪肝）的相关研究[1-4]。
在体外，Neurotensin（1μM）处理MKN-1和MKN-45胃癌细胞5分钟。Neurotensin通过NTR1受体激活基质金属蛋白酶-9（MMP-9）的表达和活性，促进细胞侵袭和迁移[5]。Neurotensin（0.1μM）处理Calu-3、NCI-H358和NCI-H441非小细胞肺癌细胞5分钟。Neurotensin显著增加HER3酪氨酸磷酸化，同时促进EGFR/HER3和HER2/HER3异源二聚体形成[6]。
在体内，Neurotensin（1pg-25ng；单次注射）侧脑室注射于CD-1小鼠。Neurotensin显著拮抗了吗啡诱导的镇痛作用[7]。Neurotensin（10μg；单次注射）鞘内注射于C57BL/6小鼠。Neurotensin显著减轻了热痛和福尔马林诱导的疼痛[8]。