Neuromedin U-25 (human) is a 25-amino-acid endogenous peptide with potent smooth muscle contractile activity [1]. Neuromedin U-25 can bind to the conserved binding pocket of two subtypes of the Neuromedin U receptors, thereby activating Gq/11[2]. Neuromedin U-25 has been widely used in the regulation of appetite and the control of energy balance[3].
In vitro, Neuromedin U-25 treatment for 4 hours significantly increased the mRNA levels of IL-5 and IL-13 in Th2 cells, with EC50 values of 3.5nM and 4.5nM respectively[4].
References:
[1] Mitchell J D, Maguire J J, Kuc R E, et al. Expression and vasoconstrictor function of anorexigenic peptides neuromedin U-25 and S in the human cardiovascular system[J]. Cardiovascular research, 2009, 81(2): 353-361.
[2] You C, Zhang Y, Xu P, et al. Structural insights into the peptide selectivity and activation of human neuromedin U receptors[J]. Nature Communications, 2022, 13(1): 2045.
[3] Martinez V G, O'Driscoll L. Neuromedin U: a multifunctional neuropeptide with pleiotropic roles[J]. Clinical chemistry, 2015, 61(3): 471-482.
[4] Ye Y, Luo J, Zeng N, et al. Neuromedin U promotes human type 2 immune responses[J]. Mucosal Immunology, 2022, 15(5): 990-999.
Neuromedin U-25 (human)是一种由25个氨基酸组成的内源性肽，具有强效的平滑肌收缩活性[1]。Neuromedin U-25能结合至神经调节肽U受体的两个亚型的保守结合口袋，从而激活Gq/11蛋白[2]。Neuromedin U-25已广泛应用于食欲调节与能量平衡控制[3]。
在体外，使用Neuromedin U-25处理Th2细胞4小时，可显著提高IL-5和IL-13的mRNA水平，EC50值分别为3.5nM和4.5nM[4]。