Bullatine B is a diterpene alkaloid that has been found in Aconitum and has diverse biological activities.1,2 It inhibits voltage-gated sodium channel 1.7 (Nav1.7) in HEK293 cells expressing the human channel (IC50 = 25.8 nM) and scavenges DPPH radicals by 65.3% at 1 mM. Bullatine B (10 mg/kg) increases the mechanical pain threshold in the von Frey test in streptozotocin-induced diabetic, but not non-diabetic, mice.1
1.Nakatani, Y., Negoro, K., Yamauchi, M., et al.Neoline, an active ingredient of the processed aconite root in Goshajinkigan formulation, targets Nav1.7 to ameliorate mechanical hyperalgesia in diabetic miceJ. Ethnopharmacol.259112963(2020) 2.Begum, S., Ali, A., Ahman, W., et al.Pharmacologically active C-19 diterpenoid alkaloids from the aerial parts of Aconitum laeve royleRec. Nat. Prod.8(2)83-92(2014)