Mefenamic acid-d4 is intended for use as an internal standard for the quantification of mefenamic acid by GC- or LC-MS. Mefenamic acid is a non-steroidal anti-inflammatory drug (NSAID) and a COX-2 inhibitor.1 It binds to COX-2 (Kd = 4 nM) and inhibits COX-2-dependent oxygenation of arachidonic acid in vitro (Ki = 10 ?M). Mefenamic acid (30 mg/kg) reduces acetic acid-induced writhing in rats.2 It inhibits increases in skin thickness in a mouse model of delayed-type hypersensitivity induced by dinitrochlorobenzene (DNBC).2 Mefenamic acid also reduces the antibody response to sheep red blood cells in mice.
1.Prusakiewicz, J.J., Duggan, K.C., Rouzer, C.A., et al.Differential sensitivity and mechanism of inhibition of COX-2 oxygenation of arachidonic acid and 2-arachidonoylglycerol by ibuprofen and mefenamic acidBiochemistry48(31)7353-7355(2009) 2.Roszkowski, A.P., Rooks, W.H., Tomolonis, A.J., et al.Anti-inflammatory and analgetic properties of d-2-(6'-methoxy-2'-naphthyl)-propionic acid (naproxen)J. Pharmacol. Exp. Ther.179(1)114-123(1971)