Lauroyl coenzyme A (lauroyl-CoA) is a thioester of lauric acid and CoA . It is produced during isomerase-dependent β-oxidation of oleic acid in isolated rat heart mitochondria.1 Lauroyl-CoA is a substrate for acyl-CoA thioesterase in isolated human skin fibroblasts and has been used as a substrate in a high-throughput assay to measure fatty acid synthesis in S. aureus, B. subtilis, and E. coli.2,3
|1. Ren, Y., and Schulz, H. Metabolic functions of the two pathways of oleate β-oxidation double bond metabolism during the β-oxidation of oleic acid in rat heart mitochondria. J. Biol. Chem. 278(1), 111-116 (2003).|2. Hunt, M.C., Ruiter, J., Mooyer, P., et al. Identification of fatty acid oxidation disorder patients with lowered acyl-CoA thioesterase activity in human skin fibroblasts. Eur. J. Clin. Invest. 35(1), 38-46 (2005).|3. Kodali, S., Galgoci, A., Young, K., et al. Determination of selectivity and efficacy of fatty acid synthesis inhibitors. J. Biol. Chem. 280(2), 1669-1677 (2005).