Biotin-XX Tyramide Reagent is a biotin derivative for enzyme-mediated tyramide signal amplification (TSA) . Biotin-XX Tyramide Reagent utilizes horseradish peroxidase (HRP) to catalyze the covalent deposition of a biotin label adjacent to an immobilized enzyme. The deposited label can be detected with a streptavidin conjugate for imaging under bright field or fluorescence microscopy. The labeling reaction is fast (less than 10 minutes) [1]. Biotin-XX Tyramide Reagent can be used to increase the sensitivity of immunohistochemistry [2].
References:
[1] Hopman AH, Ramaekers FC, Speel EJ. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7.
[2] Freedman LJ, Maddox MT. A comparison of anti-biotin and biotinylated anti-avidin double-bridge and biotinylated tyramide immunohistochemical amplification. J Neurosci Methods. 2001 Nov 15;112(1):43-9.
Biotin-XX Tyramide Reagent是一种酶介导的酪胺信号放大（TSA）的生物素衍生物。利用辣根过氧化物酶（HRP）催化生物素标记物与固定化酶相邻的共价沉积，可用链霉亲和素缀合物检测沉积标记，用于在明场或荧光显微镜下成像，标记反应速度快（少于10分钟）[1]。常用于提高免疫组织化学灵敏度[2]。