GlpBio

HT-2 Toxin

目录号: GC18185纯度: >98.00%同义词: HT-2毒素
HT-2 Toxin是 T-2 Toxin的三萜真菌代谢产物，可抑制真核细胞的蛋白质合成，导致细胞凋亡、免疫抑制和组织损伤。
HT-2 Toxin
Cas No.: 26934-87-2
规格价格库存数量操作
1mg¥3,236.00现货
1
5mg¥13,689.00现货
1
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产品描述 Description

HT-2 Toxin, a triterpenoid fungal metabolite of T-2 toxin, inhibits protein synthesis in eukaryotic cells, resulting in apoptosis, immunosuppression, and tissue damage [1-3].

In porcine brain capillary endothelial cells, the cell viability of cells incubated with HT-2 Toxin (1nM-10µM; 24h, 48h) decreased in a concentration-dependent manner [4]. In porcine oocytes, exposure to HT-2 Toxin (10nM, 50nM, 100nM; 44h) causes oxidative stress induced apoptosis/autophagy [5]. In human chondrocytes, exposure to HT-2 Toxin (20ng/mL; 48h) significantly reduced chondrocyte viability [6]. In porcine oocytes, HT-2 Toxin (100nM; 48h) impairs porcine oocyte in vitro maturation through disruption of endomembrane system [7].

In C57BL/6J mice, the body weight of mice exposed to HT-2 Toxin (1.6mg/kg, 3.2mg/kg; ig; 4 weeks) decreased in a dose-dependent manner [8]. In rat, HT-2 Toxin (0.01mg/kg, 0.1mg/kg, 0.5mg/kg, 1mg/kg; ip; 96h) elicited anorectic effects of varying durations in a dose-dependent manner [9].

References:
[1]. Nathanail AV, Varga E, Meng-Reiterer J, et al. Metabolism of the Fusarium mycotoxins T-2 toxin and HT-2 toxin in wheat. Journal of agricultural and food chemistry. 2015 Sep 9; 63(35): 7862-7872.
[2]. Meneely J, Greer B, Kolawole O, et al. T-2 and HT-2 toxins: toxicity, occurrence and analysis: a review. Toxins. 2023 Jul 29; 15(8): 481.
[3]. Zhu CC, Zhang Y, Duan X, et al. Toxic effects of HT-2 toxin on mouse oocytes and its possible mechanisms. Archives of toxicology. 2016 Jun; 90: 1495-1505.
[4]. Weidner M, Hüwel S, Ebert F, et al. Influence of T-2 and HT-2 toxin on the blood-brain barrier in vitro: new experimental hints for neurotoxic effects. PloS one. 2013 Mar 27; 8(3): e60484.
[5]. Zhang Y, Han J, Zhu CC, et al. Exposure to HT-2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes. Scientific reports. 2016 Sep 23; 6(1): 33904.
[6]. Yu FF, Lin XL, Wang X, et al Comparison of apoptosis and autophagy in human chondrocytes induced by the T-2 and HT-2 toxins. Toxins. 2019 May 8; 11(5): 260.
[7]. Li JR, Wu SL, Hu LL, et al. HT-2 toxin impairs porcine oocyte in vitro maturation through disruption of endomembrane system. Theriogenology. 2024 Sep 15; 226: 286-293.
[8]. Hao S, Yao C, Meng P, et al. The spinal consequences of HT-2 toxin and selenium deficiency during bone maturation in mice. Mycotoxin research. 2025 Feb; 41(1): 77-91.
[9]. Zhang J, Zhang H, Liu S, et al. Comparison of anorectic potencies of type A trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and neosolaniol. Toxins. 2018 Apr 29; 10(5): 179.

HT-2 Toxin是 T-2 Toxin的三萜真菌代谢产物，可抑制真核细胞的蛋白质合成，导致细胞凋亡、免疫抑制和组织损伤 [1-3]。

在猪脑毛细血管内皮细胞中，与HT-2 Toxin（1nM-10µM；24h、48h）孵育的细胞活力呈浓度依赖性降低 [4]。在猪卵母细胞中，暴露于HT-2 Toxin（10nM、50nM、100nM；44h）可导致氧化应激诱导的细胞凋亡/自噬 [5]。在人软骨细胞中，暴露于HT-2 Toxin（20ng/mL；48h）可显著降低软骨细胞活力 [6]。在猪卵母细胞中，HT-2 Toxin（100nM；48h）通过破坏内膜系统阻碍猪卵母细胞体外成熟 [7]。

在C57BL/6J小鼠中，暴露于HT-2 Toxin（1.6mg/kg、3.2mg/kg；ig；4周）的小鼠体重呈剂量依赖性下降 [8]。在大鼠中，HT-2 Toxin（0.01mg/kg、0.1mg/kg、0.5mg/kg、1mg/kg；ip；96h）以剂量依赖性方式引起不同持续时间的厌食效应 [9]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Cell lines

Porcine brain capillary endothelial cells

Preparation Method

Porcine brain capillary endothelial cells were seeded on Collagen-G-coated 96-well plates (250,000cells/cm2) and cultured according to the treatment on Transwell® filter systems (DIV 2: plating medium replaced by serum-free medium on DIV 4 for 2 days). Incubation with various T-2 and HT-2 Toxin concentrations (1nM-10µM) was carried out over 24h and 48h following the addition of the WST-8 solution [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] and incubation according to the manufacturer's manual.

Reaction Conditions

1nM-10µM; 24h, 48h

Applications

The cell viability of cells incubated with HT-2 Toxin decreased in a concentration-dependent manner.

Animal experiment [2]:

Animal models

C57BL/6J mice

Preparation Method

Male C57BL/6J mice were subjected to subchronic toxin exposure. A total of 48 mice were divided into six groups, based on toxin concentrations determined in preliminary studies. The groups included a control group, a low selenium diet group, two groups exposed to different concentrations of HT-2 Toxin (1.6mg/kg and 3.2mg/kg), and two groups combining a low selenium diet with each of these HT-2 Toxin dosages. Over a 4-week period, all mice received their designated treatments via oral gavage, mimicking typical clinical and environmental exposure scenarios.

Dosage form

1.6mg/kg, 3.2mg/kg; ig; 4 weeks

Applications

The body weight of mice exposed to HT-2 Toxin decreased in a dose-dependent manner.

References:
[1]. Weidner M, Hüwel S, Ebert F, et al. Influence of T-2 and HT-2 toxin on the blood-brain barrier in vitro: new experimental hints for neurotoxic effects. PloS one. 2013 Mar 27; 8(3): e60484.
[2]. Hao S, Yao C, Meng P, et al. The spinal consequences of HT-2 toxin and selenium deficiency during bone maturation in mice. Mycotoxin research. 2025 Feb; 41(1): 77-91.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
26934-87-2
同义词
HT-2毒素
化学名
12,13-epoxy-trichothec-9-ene-3α,4β,8α,15-tetrol, 15-acetate 8-(3-methylbutanoate)
SMILES
CC1=C[C@]2([H])[C@]([C@]([C@H](O)[C@H]3O)(C)[C@@]4(OC4)[C@]3([H])O2)(COC(C)=O)C[C@@H]1OC(CC(C)C)=O
分子式
C22H32O8
分子量
424.5 g/mol
溶解性
Dichloromethane: 5 mg/ml,DMSO: soluble,Ethanol: soluble
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

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