Histatin 5

目录号: GC31898纯度: >98%同义词: 富组蛋白5

Histatin 5可抑制基质金属蛋白酶MMP-2和MMP-9的活性，IC₅₀值分别为0.57μM和0.25μM。

Cas No.: 115966-68-2

规格	价格	库存	数量
500μg	¥1,080.00	现货	1
1mg	¥1,620.00	现货	1
5mg	¥5,670.00	现货	1
10mg	¥8,910.00	现货	1

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Nature
641, 529–536 (2025)
Nature
628, 630–638 (2024)
Nature
632, 686–694 (2024)
Nature
618, 1017–1023 (2023)
Nature
610, 366–372 (2022)
Cell
187(9):2288-2304 (2024)
Cell
183(7):1867-1883 (2020)
Science
388(6745) (2025)
Science
387(6739) (2025)
Science
387(6734) (2025)
Cell Research
35, 97–116 (2025)
Cell Research
34, 683–706 (2024)
Cell Research
33, 273–287 (2023)
Cell Research
33, 546–561 (2023)
Cell Research
33, 904–922 (2023)
Cell Research
31, 1291–1307 (2021)

产品描述 Description

Histatin 5 suppresses the activities of matrix metalloproteinases MMP-2 and MMP-9 with IC₅₀ values of 0.57 and 0.25μM, respectively^[1]. Histatin 5 significantly inhibited the growth of a variety of Candida species by binding to Candida cell wall proteins (Ssa1/2) and glycans with MIC₅₀ values of 10-20μg/ml^[2]. Histatin 5 has been widely used in metal ion binding studies and antifungal studies^[3].

In vitro, Histatin 5 at a concentration of 3200μg/mL for 24 hours was significantly cytotoxic to human gingival fibroblasts and dramatically inhibited cell viability^[4]. Treatment of human gingival fibroblasts with 10μg/ml Histatin 5 for 20 minutes inhibited the production of inflammatory cytokines (IL-6 and IL-8) induced by P. gingivalis and lipopolysaccharides^[5].

In vivo, Histatin 5 gel at a dose of 100μg/mL three times daily applied to the tongues of mice for three days prevented the occurrence of oral ulcers and significantly inhibited Candida albicans^[6]. Administration of Histatin 5 (80μM) to the cornea three times daily for one day promoted wound healing in a mouse corneal injury model^[7].

References:
[1] Gusman H, Travis J, Helmerhorst E J, et al. Salivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease[J]. Infection and immunity, 2001, 69(3): 1402-1408.
[2] Puri S, Edgerton M. How does it kill?: understanding the candidacidal mechanism of salivary histatin 5[J]. Eukaryotic cell, 2014, 13(8): 958-964.
[3] Zolin G V S, Fonseca F H, Zambom C R, et al. Histatin 5 metallopeptides and their potential against Candida albicans pathogenicity and drug resistance[J]. Biomolecules, 2021, 11(8): 1209.
[4] Moffa E B, Mussi M C M, Xiao Y, et al. Histatin 5 inhibits adhesion of C. albicans to reconstructed human oral epithelium[J]. Frontiers in Microbiology, 2015, 6: 885.
[5] Imatani T, Kato T, Minaguchi K, et al. Histatin 5 inhibits inflammatory cytokine induction from human gingival fibroblasts by Porphyromonas gingivalis[J]. Oral microbiology and immunology, 2000, 15(6): 378-382.
[6] Kong E F, Tsui C, Boyce H, et al. Development and in vivo evaluation of a novel histatin-5 bioadhesive hydrogel formulation against oral candidiasis[J]. Antimicrobial agents and chemotherapy, 2016, 60(2): 881-889.
[7] Shah D, Son K N, Kalmodia S, et al. Wound healing properties of histatin-5 and identification of a functional domain required for histatin-5-induced cell migration[J]. Molecular Therapy Methods & Clinical Development, 2020, 17: 709-716.

Histatin 5可抑制基质金属蛋白酶MMP-2和MMP-9的活性，IC₅₀值分别为0.57μM和0.25μM^[1]。Histatin 5能通过结合念珠菌的细胞壁蛋白（Ssa1/2）和多糖，显著抑制多种念珠菌的生长，MIC₅₀值为10-20μg/ml^[2]。Histatin 5已广泛应用于金属离子结合研究和抗真菌研究^[3]。

在体外，使用3200μg/mL浓度的Histatin 5处理人牙龈成纤维细胞 24 小时，可显著诱导细胞毒性并大幅降低细胞活力^[4]。以10μg/ml浓度的Histatin 5 处理人牙龈成纤维细胞20分钟，能抑制牙龈卟啉单胞菌和脂多糖诱导的炎症细胞因子（IL-6和IL-8）产生^[5]。

在体内，在小鼠舌面涂抹100μg/mL的Histatin 5凝胶（每日3次，连续3天），可预防口腔溃疡的发生并显著抑制白色念珠菌生长^[6]。在小鼠角膜损伤模型中，每日3次局部施用80μM的Histatin 5（持续1天），可促进角膜伤口愈合^[7]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:
Cell lines	Human gingival fibroblast cells
Preparation Method	The cytotoxic effect of Histatin 5 was assessed on human gingival fibroblast cells grown in Dulbeccco's modified Eagle medium supplemented with antibiotic-fungal solution and 10% v/v fetal bovine serum. The incubation temperature was 37°C with 5% CO₂ in air and 95% air concentration. Cells were cultured to confluence (90%) and removed with trypsin (0.05%)/EDTA (0.02%) in 1×PBS. Medium was added to inactivate trypsin, and cells were then centrifuged at 2000rpm for 5min, resuspended, and re-seeded. The medium was changed two to three times a week. Total viable cell counts were performed in an incubator. Cell suspensions containing 2.0×10⁴ cells/ml were incubated in 24-well plates for 48 hours at 37°C in a humidified environment with 5% CO₂. At the end of the culture, the medium was discarded, and adherent cells remained at the bottom of the plate. Gradient dilutions of Histatin 5 (100, 200, 400, 800, 1600, 3200, 6400, and 12800μg/mL) were performed with fresh medium. The plates were incubated at 37°C in an atmosphere of 5% CO₂ and 95% air for 24 hours. MTT assay was used to measure mitochondrial dehydrogenase activity. After the cells were grown in the control or test medium for 24h, 100μL of MTT stock solution was added to each well. The culture plates were incubated at 37°C in 5% CO₂ for 4 hours. At the end of incubation, the cultures were removed from the incubator and 100μL of MTT solubilization solution was added to dissolve the resulting formazan crystals. The plates were then shaken until the crystals were completely dissolved, and the absorbance was measured at a wavelength of 570nm by spectrophotometry; all experiments were performed three times.
Reaction Conditions	100, 200, 400, 800, 1600, 3200, 6400, and 12800μg/mL; 24h
Applications	Histatin 5 at concentrations above 3200μg/mL was significantly cytotoxic, resulting in an apparent reduction in gingival fibroblast cell viability.
Animal experiment [2]:
Animal models	C57BL/6 mice
Preparation Method	The C57BL/6J mice aged between twelve and nineteen weeks received anesthesia through an intraperitoneal injection containing ketamine at 100mg/kg and xylazine at 5mg/kg. A circular 2.0-mm central epithelial segment was delineated with a 2-mm biopsy punch following 2 drops of topical 0.5% proparacaine, then removed with a brush. Histatin 5 (80μM), SHRGY (80μM) was applied to the cornea three times a day during the treatment (n = 7) or the control (n = 7) group. At 0, 18, and 24h, corneas were stained with fluorescein and imaged using a photo-slit lamp with a camera. The remaining wound areas were measured with the ImageJ software at each indicated time point and were compared with the baseline wound area for each mouse. The percentage of the remaining wound area was calculated for each time point.
Dosage form	80μM for three times a day; apply to the cornea
Applications	Histatin 5 treatment significantly promoted wound healing in a mouse corneal injury model.
References: [1] Moffa E B, Mussi M C M, Xiao Y, et al. Histatin 5 inhibits adhesion of C. albicans to reconstructed human oral epithelium[J]. Frontiers in Microbiology, 2015, 6: 885. [2] Shah D, Son K N, Kalmodia S, et al. Wound healing properties of histatin-5 and identification of a functional domain required for histatin-5-induced cell migration[J]. Molecular Therapy Methods & Clinical Development, 2020, 17: 709-716.

产品文档 Product Documents

Purity:>98%

COA SDS Data Sheet

化学性质Chemical Properties

CAS 号

115966-68-2

同义词

富组蛋白5

SMILES

Asp-Ser-His-Ala-Lys-Arg-His-His-Gly-Tyr-Lys-Arg-Lys-Phe-His-Glu-Lys-His-His-Ser-His-Arg-Gly-Tyr

分子式

C₁₃₃H₁₉₅N₅₁O₃₃

分子量

3036.29 g/mol

溶解性

Soluble in DMSO

保存条件

Store at -20°C

General tips

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。

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