Hexahydrocurcumin is a natural product and an active metabolite of curcumin.1 It reduces prostaglandin E2 (PGE2) production stimulated by phorbol 12-myristate 13-acetate by 37% in human colonic epithelial cells when used at a concentration of 20 ?M. Hexahydrocurcumin (3.125-25 ?M) inhibits overproduction of nitric oxide induced by LPS in RAW 264.7 macrophage cells in a concentration-dependent manner.2 It does not affect LPS-induced cytokine release but inhibits LPS-induced iNOS and COX-2 upregulation and NF-κB activation when used at a concentration of 50 ?M. Hexahydrocurcumin prevents the formation of aberrant crypt foci in a dimethylhydrazine rat model of colon cancer and potentiates the effect of 5-fluorouracil .3
1.Ireson, C., Orr, S., Jones, D.J., et al.Characterization of metabolites of the chemopreventive agent curcumin in human and rat hepatocytes and in the rat in vivo, and evaluation of their ability to inhibit phorbol ester-induced prostaglandin E2 productionCancer Res.61(3)1058-1064(2001) 2.Zhao, F., Gong, Y., Hu, Y., et al.Curcumin and its major metabolites inhibit the inflammatory response induced by lipopolysaccharide: Translocation of nuclear factor-κB as potential targetMol. Med. Rep.11(4)3087-3093(2015) 3.Srimuangwong, K., Tocharus, C., Tocharus, J., et al.Effects of hexahydrocurcumin in combination with 5-fluorouracil on dimethylhydrazine-induced colon cancer in ratsWorld J. Gastroenterol.18(47)6951-6959(2012)