The Hexa His tag peptide is a short peptide composed of six consecutive histidine residues and also serves as a purification tag for fusion proteins. The Hexa His tag peptide can chelate with metal ions such as nickel and cobalt. Recombinant proteins can be efficiently purified by immobilized metal ion affinity chromatography[1]. The Hexa His tag peptide is commonly used to prepare protein mimics for nanocrystals[2].
References:
[1] Schmidt TG, Koepke J, Frank R, et al. Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin. J Mol Biol. 1996 Feb 9;255(5):753-66.
[2] Zhou W, Baneyx F. Biofabrication of ZnS:Mn luminescent nanocrystals using histidine, hexahistidine, and His-tagged proteins: a comparison study. Biochem Eng J. 2014 Aug 15;89:28-32.
Hexa His tag peptide是一种由六个连续组氨酸残基组成的短肽，也是融合蛋白的纯化标签。Hexa His tag peptide可以与镍、钴等金属离子螯合，通过固定化金属离子亲和层析高效纯化重组蛋白[1]。Hexa His tag peptide常被用于制备纳米晶体的蛋白质模拟物[2]。