S Tag Peptide, a 15-amino acid peptide derived from ribonuclease A, binds to S protein to regenerate active enzyme with a Kd of >10-9M [1]. S Tag Peptide serves as a fusion tag for recombinant proteins, facilitating detection by a rapid and sensitive homogeneous assay and by colorimetric detection in Western blots [2]. S Tag Peptide is a tunable peptide tag that allows functional proteins to be cross-linked at specific sites [3]. A protein purification S-tag fusion system can be developed by using S Tag Peptide in antibody and epitope labeling techniques, which allows for automation and makes the system easier to use in a variety of high-throughput screening scenarios [4].
References:
[1] Hus C H, Rad N F, Hackbarth J S, et al. S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells[J]. Biotechniques, 2004, 37(5): 835-839.
[2] McCormick M, Mierendorf R. S. Tag™: A multipurpose fusion peptide for recombinant proteins[J]. InNovations, 1994, 1: 4-7.
[3] Kamiya N, Tanaka T, Suzuki T, et al. S-peptide as a potent peptidyl linker for protein cross-linking by microbial transglutaminase from Streptomyces mobaraensis[J]. Bioconjugate chemistry, 2003, 14(2): 351-357.
[4] Raines R T, McCormick M, Van Oosbree T R, et al. [23] The S· tag fusion system for protein purification[M]//Methods in enzymology. Academic Press, 2000, 326: 362-376.
S Tag Peptide是一种源自ribonuclease A的15个氨基酸的肽段，与S蛋白的结合Kd值大于10-9M，从而再生出活性酶[1]。S Tag Peptide可作为重组蛋白的新型融合标签，通过快速灵敏的均相检测或Western blot中的比色检测实现检测[2]。S Tag Peptide可用作可调控的肽标签，实现功能蛋白的位点特异性交联[3]。S Tag Peptide可用于抗体和表位标记技术，以开发蛋白纯化S Tag融合系统，实现自动化并促进在各种高通量筛选场景中的应用[4]。