Laminin (925-933)（CDPGYIGSR）, is the sequence of Laminin on the B1 chain. YIGSR is active in promoting the adhesion of a variety of epithelial cells[1]. Laminin binds to colagen IV, heparan sulfate proteoglycan, and nidogen-entactin,and it mediates cellular interactions with this ma-trix[2].
A laminin-derived synthetic peptide, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2 (CDPGYIGSR-H2), containing an active site for cell binding inhibited both angiogenesis and solid tumor growth[3].
In vitro, at concentrations of 100 and 300 µg/ml, Laminin (925-933) can stimulate the attachment of HT-1080 and CHO cells to culture plates[2].
In vitro, the oligopeptide domain Laminin (925-933) covalently linked to an agarose gel as a bioartificial 3D substrate successfully supports neurite outgrowth from dorsal root ganglia (DRG)[2]. In vitro, a 78% reduction in cell attachment was observed on films containing Laminin (925-933) in the cell plating medium[4].
In vivo experiments revealed the capability of a Laminin (925-933)-derivatized gel to enhance nerve regeneration in a transected rat dorsal root model compared to an underivatized gel, a Laminin (925-933) gel, and saline-filled nerve guidance channels[3].
References:
[1] Graf J, et al. A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor. Biochemistry. 1987 Nov 3;26(22):6896-900.
[2] Graf J, et al. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell. 1987 Mar 27;48(6):989-96.
[3] Borkenhagen M, et al. Three-dimensional extracellular matrix engineering in the nervous system. J Biomed Mater Res. 1998 Jun 5;40(3):392-400.
[4] Ranieri JP, et al. Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates. Int J Dev Neurosci. 1994 Dec;12(8):725-35.
层粘连蛋白（925-933）（CDPGYIGSR）是层粘连蛋白 B1 链上的序列。YIGSR 在促进多种上皮细胞粘附方面具有活性[1]。层粘连蛋白能与胶原 IV、硫酸肝素蛋白聚糖和核苷酸-触角蛋白结合，并介导细胞与这些物质的相互作用[2]。
Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2（CDPGYIGSR-H2）层粘连蛋白的合成肽含有细胞结合的活性位点，可抑制血管生成和实体瘤的生长[3]。
在体外，浓度为 100 和 300 µg/ml 的层粘连蛋白（925-933）可刺激 HT-1080 和 CHO 细胞附着在培养板上[2]。
在体外，寡肽结构域层粘连蛋白（925-933）共价连接到琼脂糖凝胶作为生物人工三维基底，可成功支持背根神经节（DRG）神经元的生长[2]。在体外实验中，在细胞培养介质中含有层粘连蛋白（925-933）的薄膜上观察到细胞附着减少了78%[4]。
体内实验显示，与未充分活化的凝胶、层粘连蛋白（925-933）凝胶和生理盐水填充的神经引导通道相比，层粘连蛋白（925-933）-活化的凝胶能增强横断大鼠背根模型的神经再生能力[3]。