HBC514 is a non-fluorescent HBC analog that only emits green fluorescence upon forming a tight complex with the Pepper RNA aptamer, widely used for RNA labeling, with Ex/Em wavelengths of 485nm/530nm[1]. The binding between HBC514 and the Pepper aptamer exhibits high specificity, and the complex demonstrates high signal intensity and exceptional photostability in live cells, making it suitable for live-cell imaging, RNA dynamic tracking, and CRISPR-based biosensing applications[2,3].
References:
[1] CHEN X, ZHANG D, SU N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs[J]. Nature Biotechnology, 2019, 37(11): 1287-1293.
[2] YU Q, REN K, YOU M. Genetically encoded RNA nanodevices for cellular imaging and regulation[J]. Nanoscale, 2021, 13(17): 7988-8003.
[3] YUAN D, HE H, SONG W, et al. Allosteric genetically encoded biosensor for spatiotemporal monitoring of endogenous RNA dynamics in living cells[J]. Proceedings of the National Academy of Sciences, 2025, 122(7): e2409309122.
HBC514是一种与Pepper RNA适配体形成紧密复合物后才能发出绿色荧光的非荧光HBC类似物，被广泛用于RNA标记，Ex/Em为485nm/530nm[1]。HBC514与Pepper适配体的结合具有高特异性，且复合物在活细胞中表现出良好的亮度和稳定性，适用于活细胞成像、RNA动态观察和基于CRISPR的生物感测等领域[2,3]。