G13KS is a deubiquitinase UCHL1 ligand and inhibitor. G13KS inhibits recombinant and cellular UCHL1. G13KS reduces levels of monoubiquitin in human glioblastoma cells. GK13S is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
GK13S (0-1 μM, 1 h) inhibits recombinant UCHL1 with an IC50 of 50 nM[1].GK13S (1-10 μM, 24 h) inhibits cellular UCHL1 in HEK293 cells[1].GK13S (5 μM, 72 h) does not impair HEK293 cell growth[1].GK13S (5 μM, 48 h) reduces ubiquitin levels in U-87 MG cells[1].
References:
[1]. Grethe C, et al. Structural basis for specific inhibition of the deubiquitinase UCHL1. Nat Commun. 2022 Oct 10;13(1):5950.