1,6-Diphenyl-1,3,5-hexatriene is a fluorescent probe for lipid monolayers and bilayer membranes (with maximum excitation and emission wavelengths of 360/430nm). 1,6-Diphenyl-1,3,5-hexatriene can be localized in the hydrocarbon region of membrane lipids, exhibiting almost no fluorescence in water, but its fluorescence increases significantly upon membrane insertion, thereby enabling the detection of membrane viscosity, polarity, and lipid order. 1,6-Diphenyl-1,3,5-hexatriene can be used in studies of membrane fluidity, lipid droplet fluorescence detection, determination of surfactant critical micelle concentration, and reporting of bacterial spore membrane fluidity, among other related research[1-4].
References:
[1] He W. DPH Probe Method for Liposome-Membrane Fluidity Determination. Methods Mol Biol. 2023;2622:241-244.
[2] Parasassi T, Sapora O, Giusti AM, et al. Alterations in erythrocyte membrane lipids induced by low doses of ionizing radiation as revealed by 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime. Int J Radiat Biol. 1991 Jan;59(1):59-69.
[3] Bouchy M, Donner M, André JC. Evolution of fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) during the labelling of living cells. Exp Cell Res. 1981 May;133(1):39-46.
[4] Ibrahim H, Jurcic K, Wang JS, et al. 1,6-Diphenyl-1,3,5-hexatriene (DPH) as a Novel Matrix for MALDI MS Imaging of Fatty Acids, Phospholipids, and Sulfatides in Brain Tissues. Anal Chem. 2017 Dec 5;89(23):12828-12836.
1,6-Diphenyl-1,3,5-hexatriene是一种脂质单分子层和双分子层膜的荧光探针（最大激发光和发射光波长为360/430nm）。1,6-Diphenyl-1,3,5-hexatriene可定位在膜脂质的烃区，在水中几乎无荧光，但嵌入膜后荧光显著增强，从而用于检测膜粘度、极性和脂质顺序。1,6-Diphenyl-1,3,5-hexatriene可用于膜流动性研究、脂滴荧光检测、表面活性剂临界胶束浓度测定以及细菌孢子膜流动性报告等相关研究[1-4]。