Bis(2-ethylhexyl) phthalate, commonly referred to as DEHP, is a synthetic compound primarily used as a plasticizer[1]. Exposure to Bis(2-ethylhexyl) phthalate can have various adverse effects on organisms, such as impaired reproduction and development, cancer, altered metabolism, and neurological and behavioral disorders[2].
In vitro, Bis(2-ethylhexyl) phthalate (50μM and 150μM; 48h) and MEHP induce glucose and lipid metabolic abnormalities in hepatocytes[3]. Bis(2-ethylhexyl) phthalate (0-80µM; 48h) significantly inhibited mouse TM3 Leydig cells viability and induced cell apoptosis[4].
In vivo, Bis(2-ethylhexyl) phthalate (5/10mg/kg/day; 16 weeks; p.o.) exposure elevates plasma glucose and disrupts hepatic lipid metabolism in mice[3]. Chronic, low-level exposure to Bis(2-ethylhexyl) phthalate (37µg/kg; 34 days; p.o.) enhances OVA-induced allergic lung inflammation in mice[5]. Prenatal exposure to Bis(2-ethylhexyl) phthalate (20 and 200μg/kg/day and 200, 500 and 750mg/kg/day; p.o.) disrupts ovarian function in a transgenerational manner in female mice[6].
References:
[1] Gaur K, Varshney H, Subhan I, Fatima J, Jyoti S, Siddique YH. Evaluation of Bis(2-ethylhexyl) phthalate toxicity on the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9. Food Chem Toxicol. 2024 Feb;184:114425.
[2] Martínez-Razo LD, Martínez-Ibarra A, Vázquez-Martínez ER, Cerbón M. The impact of Di-(2-ethylhexyl) Phthalate and Mono(2-ethylhexyl) Phthalate in placental development, function, and pathophysiology. Environ Int. 2021 Jan;146:106228.
[3] Wei X, Yang D, Zhang B, Fan X, Du H, Zhu R, Sun X, Zhao M, Gu N. Di-(2-ethylhexyl) phthalate increases plasma glucose and induces lipid metabolic disorders via FoxO1 in adult mice. Sci Total Environ. 2022 Oct 10;842:156815.
[4] Sun Y, Shen J, Zeng L, Yang D, Shao S, Wang J, Wei J, Xiong J, Chen J. Role of autophagy in di-2-ethylhexyl phthalate (DEHP)-induced apoptosis in mouse Leydig cells. Environ Pollut. 2018 Dec;243(Pt A):563-572.
[5] Tseng HH, Li CY, Wu ST, Su HH, Wong TH, Wu HE, Chang YW, Huang SK, Tsai EM, Suen JL. Di-(2-ethylhexyl) Phthalate Promotes Allergic Lung Inflammation by Modulating CD8α+ Dendritic Cell Differentiation via Metabolite MEHP-PPARγ Axis. Front Immunol. 2022 May 19;13:581854.
[6] Rattan S, Brehm E, Gao L, Niermann S, Flaws JA. Prenatal exposure to di(2-ethylhexyl) phthalate disrupts ovarian function in a transgenerational manner in female mice. Biol Reprod. 2018 Jan 1;98(1):130-145.
Bis(2-ethylhexyl) phthalate，通常称为DEHP，是一种合成化合物，主要用作增塑剂[1]。接触Bis(2-ethylhexyl) phthalate会对生物体产生各种不良影响，如生殖和发育受损、癌症、代谢改变以及神经和行为障碍[2]。
在体外实验中，Bis(2-ethylhexyl) phthalate (50µM和150µM; 48小时)和MEHP能够诱导肝细胞出现葡萄糖和脂质代谢异常[3]。此外，Bis(2-ethylhexyl) phthalate (0-80µM; 48小时)显著抑制了小鼠TM3 Leydig细胞的活性，并诱导了细胞凋亡[4]。
在体内实验中，Bis(2-ethylhexyl) phthalate (5/10mg/kg/天; 16周; 口服）暴露能够提高小鼠的血浆葡萄糖水平，并破坏肝脏脂质代谢[3]。长期低水平暴露于Bis(2-ethylhexyl) phthalate (37µg/kg; 34天; 口服)能够增强卵清蛋白(OVA)诱导的小鼠过敏性肺部炎症[5]。在母体孕期暴露于Bis(2-ethylhexyl) phthalate (20和200µg/kg/天以及200, 500和750mg/kg/天; 口服)能够以跨代方式破坏雌性小鼠的卵巢功能[6]。