Dihydrokaempferol (Aromadendrin) is a naturally occurring flavonoid compound with biological activities such as free radical scavenging, anti-inflammatory and anti-tumor[1, 2]. Dihydrokaempferol can activate the SIRT1 pathway, thereby activating cell autophagy, reducing oxidative stress damage and inhibiting inflammatory response[3].
In vitro, Dihydrokaempferol (10, 30μM) treatment of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) for 48h concentration-dependently inhibited cell viability, induced cell apoptosis and was able to express apoptosis-related proteins[4]. Dihydrokaempferol (0-200μM) pretreatment of RAW264.7 macrophages for 1h inhibited LPS-induced NO and PGE2 production in a concentration-dependent manner[5]. Dihydrokaempferol (20μM) pretreatment of SH-SY5y neuronal cells for 1h inhibited methamphetamine (METH)-induced cytotoxicity and enhanced cell confluence[6].
In vivo, oral administration of Dihydrokaempferol (80mg/kg) to treat severe acute pancreatitis (SAP) mice regulated the Keap1/Nrf2 pathway, reduced oxidative stress damage, and improved SAP-induced pancreatic damage[7].
References:
[1] Kim J K, Park S U. Recent studies on kaempferol and its biological and pharmacological activities[J]. EXCLI journal, 2020, 19: 627-634.
[2] Wang S H, Hu Y L, Liu T X. Plant distribution and pharmacological activity of flavonoids[J]. Tradit. Med. Res, 2019, 4: 269-287.
[3] Zhang J, Hu C, Li X, et al. Protective effect of dihydrokaempferol on acetaminophen-induced liver injury by activating the SIRT1 pathway[J]. The American Journal of Chinese Medicine, 2021, 49(03): 705-718.
[4] Zhang Y, Yan G, Sun C, et al. Apoptosis effects of dihydrokaempferol isolated from Bauhinia championii on synoviocytes[J]. Evidence‐Based Complementary and Alternative Medicine, 2018, 2018(1): 9806160.
[5] Lee J W, Kim N H, Kim J Y, et al. Aromadendrin inhibits lipopolysaccharide-induced nuclear translocation of NF-κB and phosphorylation of JNK in RAW 264.7 macrophage cells[J]. Biomolecules & Therapeutics, 2013, 21(3): 216.
[6] Lee H S, Kim E N, Jeong G S. Aromadendrin protects neuronal cells from methamphetamine-induced neurotoxicity by regulating endoplasmic reticulum stress and PI3K/Akt/mTOR signaling pathway[J]. International Journal of Molecular Sciences, 2021, 22(5): 2274.
[7] Liang X, Hu C, Liu C, et al. Dihydrokaempferol (DHK) ameliorates severe acute pancreatitis (SAP) via Keap1/Nrf2 pathway[J]. Life Sciences, 2020, 261: 118340.
Dihydrokaempferol（Aromadendrin）是一种天然存在的黄酮类化合物，具有清除自由基、抗炎、抗肿瘤等生物活性[1, 2]。Dihydrokaempferol能够激活SIRT1通路，从而激活细胞自噬、减少氧化应激损伤并抑制炎症反应[3]。
在体外，Dihydrokaempferol（10, 30μM）处理类风湿关节炎成纤维样滑膜细胞（RA-FLSs）48h，浓度依赖性地抑制了细胞活力，诱导了细胞凋亡并且能够凋亡相关蛋白表达[4]。Dihydrokaempferol（0-200μM）预处理RAW264.7巨噬细胞细胞1h，以浓度依赖性方式抑制了LPS诱导的NO和PGE2产生[5]。Dihydrokaempferol（20μM）预处理SH-SY5y神经元细胞1h，抑制了甲基苯丙胺（METH）诱导的细胞毒性，增强了细胞的汇合度[6]。
在体内，Dihydrokaempferol（80mg/kg）通过口服治疗重症急性胰腺炎（SAP）小鼠，调节了Keap1/Nrf2通路，减少了氧化应激损伤，改善了SAP引起的胰腺损伤[7]。