3-Hydroxyflavone is a synthetic flavonol.1 It reduces nicotine-induced cytotoxicity and production of reactive oxygen species (ROS) in NRK-52E cells when used at a concentration of 20 ?M. 3-Hydroxyflavone (30 and 50 ?M) inhibits the motility of U2OS, and the invasiveness of 143B, osteosarcoma cells.2 It reduces tumor growth in a 143B mouse xenograft model when administered at doses of 10 and 20 mg/kg and reduces the formation of pulmonary metastases in a 143B mouse model of metastasis at 20 mg/kg. 3-Hydroxyflavone also reduces acetic acid-induced writhing in mice (ED50 = 55 mg/kg).3
1.Sengupta, B., Sahihi, M., Dehkhodaei, M., et al.Differential roles of 3-hydroxyflavone and 7-hydroxyflavone against nicotine-induced oxidative stress in rat renal proximal tubule cellsPLoS One12(6)e0179777(2014) 2.Lu, K.-H., Chen, P.-N., Hsieh, Y.-H., et al.3-Hydroxyflavone inhibits human osteosarcoma U2OS and 143B cells metastasis by affecting EMT and repressing u-PA/MMP-2 via FAK-Src to MEK/ERK and RhoA/MLC2 pathways and reduces 143B tumor growth in vivoFood Chem. Toxicol.97177-186(2016) 3.Thirugnanasambantham, P., Viswanathan, S., Mythirayee, C., et al.Analgesic activity of certain flavone derivatives: A structure-activity studyJ. Ethnopharmacol.28(2)207-214(1990)