Tetracycline (TC) is a broad-spectrum antibiotic with oral activity. It is effective against a variety of bacteria, including gram-positive and gram-negative bacteria, chlamydia, mycoplasma, and Rickettsia. It is used in the study of infections and can induce gene expression in the corresponding system[1-4].
Tetracycline (6.25-250 μM) was dose-dependent on NO synthetase activity and nitrite formation in J774 cells[5].
Tetracycline treatment delays the onset and slows the progression of diabetes in human amylin/islet amyloid polypeptide (hA/hIAPP) transgenic mice. Chronic oral administration of tetracycline (0.03 mg/ml in drinking water) from weaning enhances glycemic control and extends lifespan in hemizygous mice [6]. In a dog model of acute ischemia, 4mg/kg tetracycline injected 30 min prior to the occlusion improved the functional recovery from stunning of myocardium caused by ischemia[7]. Tetracycline injection at single doses of 1.5, 10, and 20 mg/kg significantly protected mice from a lethal intraperitoneal injection of LPS[8].
References:
[1]. Platt BN, Jacobs CA, et,al. Tetracycline use in treating osteoarthritis: a systematic review. Inflamm Res. 2021 Mar;70(3):249-259. doi: 10.1007/s00011-021-01435-4. Epub 2021 Jan 29. PMID: 33512569.
[2]. Chopra I, Roberts M. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60 ; second page, table of contents. doi: 10.1128/MMBR.65.2.232-260.2001. PMID: 11381101; PMCID: PMC99026.
[3]. Roberts MC. Tetracycline therapy: update. Clin Infect Dis. 2003 Feb 15;36(4):462-7. doi: 10.1086/367622. Epub 2003 Jan 28. PMID: 12567304.
[4]. Aiba A, Nakao H. Conditional mutant mice using tetracycline-controlled gene expression system in the brain. Neurosci Res. 2007 Jun;58(2):113-7. doi: 10.1016/j.neures.2007.01.009. Epub 2007 Jan 20. PMID: 17316857.
[5]. D'Agostino P, Arcoleo F, et,al. Tetracycline inhibits the nitric oxide synthase activity induced by endotoxin in cultured murine macrophages. Eur J Pharmacol. 1998 Apr 10;346(2-3):283-90. doi: 10.1016/s0014-2999(98)00046-6. PMID: 9652371.
[6]. Aitken JF, Loomes KM, et,al. Tetracycline treatment retards the onset and slows the progression of diabetes in human amylin/islet amyloid polypeptide transgenic mice. Diabetes. 2010 Jan;59(1):161-71. doi: 10.2337/db09-0548. Epub 2009 Sep 30. PMID: 19794060; PMCID: PMC2797917.
[7]. Kagawa N, Senbonmatsu TA, et,al. Tetracycline protects myocardium against ischemic injury. Front Biosci. 2005 Jan 1;10:608-19. doi: 10.2741/1557. PMID: 15569603.
[8]. Milano S, Arcoleo F, et,al. Intraperitoneal injection of tetracyclines protects mice from lethal endotoxemia downregulating inducible nitric oxide synthase in various organs and cytokine and nitrate secretion in blood. Antimicrob Agents Chemother. 1997 Jan;41(1):117-21. doi: 10.1128/AAC.41.1.117. PMID: 8980766; PMCID: PMC163671.
Tetracycline(TC,四环素)是一种具有口服活性的广谱抗生素，可有效抑制革兰氏阳性菌、革兰氏阴性菌、衣原体、支原体和立克次体等细菌的活性。Tetracycline可用于研究感染。Tetracycline可诱导相应系统的基因表达[1-4]。
Tetracycline(6.25-250 μM)在J774细胞中剂量依赖性地降低了脂多糖刺激诱导的NO合成酶活性和亚硝酸盐的形成[5]。
Tetracycline在人胰淀素/胰岛淀粉样多肽(hA/hIAPP)转基因小鼠中可延缓糖尿病的发生和进展。断奶后长期口服Tetracycline(0.03 mg/ml drinking water)可改善半合子小鼠的血糖控制和寿命[6]。在狗急性缺血模型中，血管闭塞前30分钟注射4mg/kg Tetracycline可改善缺血引起的心肌休克的功能恢复[7]。注射单剂量Tetracycline(1.5, 10 and 20 mg/kg)的小鼠可明显避免腹腔注射致死性LPS[8]。