Jagged-1 (188-204) TFA is a fragment of the Jagged-1 (JAG-1) protein, an agonist of the neurogenic site Notch homologous protein 1 (Notch1)[1]. Jagged-1 is a Notch ligand that is highly expressed in cultured and primary multiple myeloma (MM) cells[2]. Jagged-1-mediated Notch activation can induce full maturation of human keratinocytes[3]. Jagged-1-mediated Notch activation can promote the differentiation of human limbal epithelial cells[4].
In vitro, treatment of human keratinocytes (KC) with Jagged-1 (188-204) TFA (40μM) for 72h induced the expression of loricrin and involucrin, and induced the cellular redistribution of Notch1, 3, and 4[3]. Jagged-1 (188-204) TFA (50μM) treatment of human pulmonary artery smooth muscle cells (PASMC) for 0-60min enhanced store-operated Ca2+ entry (SOCE) in the cells[5].
References:
[1] Chiappara G, Sciarrino S, Di Sano C, et al. Notch‐1 signaling activation sustains overexpression of interleukin 33 in the epithelium of nasal polyps[J]. Journal of Cellular Physiology, 2019, 234(4): 4582-4596.
[2] Jundt F, Pröbsting K S, Anagnostopoulos I, et al. Jagged1-induced Notch signaling drives proliferation of multiple myeloma cells[J]. Blood, 2004, 103(9): 3511-3515.
[3] Nickoloff B J, Qin J Z, Chaturvedi V, et al. Jagged-1 mediated activation of notch signaling induces complete maturation of human keratinocytes through NF-κB and PPARγ[J]. Cell Death & Differentiation, 2002, 9(8): 842-855.
[4] González S, Halabi M, Ju D, et al. Role of Jagged1-mediated Notch signaling activation in the differentiation and stratification of the human limbal epithelium[J]. Cells, 2020, 9(9): 1945.
[5] Yamamura H, Yamamura A, Ko E A, et al. Activation of Notch signaling by short-term treatment with Jagged-1 enhances store-operated Ca2+ entry in human pulmonary arterial smooth muscle cells[J]. American Journal of Physiology-Cell Physiology, 2014, 306(9): C871-C878.
Jagged-1 (188-204) TFA是Jagged-1（JAG-1）蛋白的一个片段，是神经原性位点Notch同源蛋白1（Notch1）的激动剂[1]。Jagged-1是一种Notch配体，在培养的和原发性多发性骨髓瘤（MM）细胞中高度表达[2]。Jagged-1介导的Notch激活能够诱导人角质形成细胞完全成熟[3]。Jagged-1介导的Notch激活能够促进人角膜缘上皮细胞的分化[4]。
在体外，Jagged-1 (188-204) TFA（40μM）处理人角质形成细胞（KC）72h，诱导了兜甲蛋白（loricrin）和外皮蛋白（involucrin）表达，诱导了Notch1、3和4的细胞再分布[3]。Jagged-1 (188-204) TFA（50μM）处理人类肺动脉平滑肌细胞（PASMC）0-60min，增强了细胞中钙库操纵的Ca2+内流（SOCE）[5]。