Glabranine是一种从Tephrosia s.p.中分离的黄酮类化合物。Glabranine通过与DENV2型E蛋白的可溶性胞外域相互作用来抑制登革热病毒。
Cas No.:41983-91-9
Sample solution is provided at 25 µL, 10mM.
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Glabranine is a flavonoid isolated from Tephrosia species. Glabranine inhibits the dengue virus by interacting with the soluble extracellular domain of the DENV2 E protein. Glabranine can be used in research related to dengue virus inhibition, as well as for Glabranine antibacterial and antioxidant activities[1].
In vitro, Glabranine (6–50µM) was used to treat LLC-MK2 cells infected with dengue virus (serotype 2) for 24 hours. Glabranine exhibited no cytotoxicity to the cells and significantly inhibited dengue virus replication[2].
References:
[1] Ismail NA, Jusoh SA. Molecular Docking and Molecular Dynamics Simulation Studies to Predict Flavonoid Binding on the Surface of DENV2 E Protein. Interdiscip Sci. 2017 Dec;9(4):499-511.
[2] Sánchez I, Gómez-Garibay F, Taboada J, et al. Antiviral effect of flavonoids on the dengue virus. Phytother Res. 2000 Mar;14(2):89-92.
Glabranine是一种从Tephrosia s.p.中分离的黄酮类化合物。Glabranine通过与DENV2型E蛋白的可溶性胞外域相互作用来抑制登革热病毒。Glabranine可用于登革热病毒抑制和抗菌抗氧化相关研究[1]。
在体外，Glabranine（6–50µM）处理登革热病毒（血清型2）感染的LLC-MK2细胞24小时，对细胞无细胞毒性，并显著抑制登革热病毒复制[2]。
| Cell experiment [1]: | |
Cell lines | LLC-MK2 cells (Rhesus monkey kidney epithelial cell line) |
Preparation Method | LLC-MK2 cells were treated with Glabranine at concentrations ranging from 6µM to 50µM for 24 hours. |
Reaction Conditions | 6µM to 50µM; 24h. |
Applications | Glabranine showed no cytotoxicity to LLC-MK2 cells at concentrations up to 50µM and did not affect cell proliferation. In dengue virus (serotype 2)-infected cells, Glabranine significantly inhibited viral replication, reducing dengue virus by 53.9% and 76.9% at concentrations of 12µM and 25µM, respectively. |
References: | |
| Cas No. | 41983-91-9 | SDF | |
| 别名 | 光甘草宁；欧亚甘草宁；裸禾蕨黄素 | ||
| Canonical SMILES | O=C1C2=C(O)C=C(O)C(C/C=C(C)\C)=C2O[C@H](C3=CC=CC=C3)C1 | ||
| 分子式 | C20H20O4 | 分子量 | 324.37 |
| 溶解度 | DMSO : 100 mg/mL (308.29 mM; Need ultrasonic) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0829 mL | 15.4145 mL | 30.829 mL |
| 5 mM | 616.6 μL | 3.0829 mL | 6.1658 mL |
| 10 mM | 308.3 μL | 1.5414 mL | 3.0829 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet