CY5 is a cyanine-containing fluorescent dye that is commonly used to label proteins, antibodies, and small molecule compounds. Its maximum excitation/emission wavelengths are 650/670nm, respectively[1]. The basic structure of CY5 is a cyclic compound with a conjugated double bond system. It has excellent fluorescence properties in the far-red region and good photostability[2]. CY5 usually exists in the form of its active ester or azide, which enables it to be labeled with biological molecules such as proteins and nucleic acids through covalent bonds[3]. The optimal molar ratio of CY5 to protein is about 10:1[4]. CY5 has a wide range of applications. It is not only suitable for biomarkers and cell imaging, but also for nucleic acid detection, protein analysis, and drug screening[5]. The molecular weight of this product is 656.81. It is for research purposes only and is not suitable for food, drugs, medical devices, or cosmetics.
References:
[1] Ferri G L, Isola J, Berger P, et al. Direct eye visualization of Cy5 fluorescence for immunocytochemistry and in situ hybridization[J]. Journal of Histochemistry & Cytochemistry, 2000, 48(3): 437-444.
[2] Yuan L, Lin W, Zheng K, et al. Far-red to near infrared analyte-responsive fluorescent probes based on organic fluorophore platforms for fluorescence imaging[J]. Chemical Society Reviews, 2013, 42(2): 622-661.
[3] Gerowska M, Hall L, Richardson J, et al. Efficient reverse click labeling of azide oligonucleotides with multiple alkynyl Cy-Dyes applied to the synthesis of HyBeacon probes for genetic analysis[J]. Tetrahedron, 2012, 68(3): 857-864.
[4] Yu H, Chao J, Patek D, et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes[J]. Nucleic Acids Research, 1994, 22(15): 3226-3232.
[5] Ntziachristos V, Schellenberger E A, Ripoll J, et al. Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V–Cy5. 5 conjugate[J]. Proceedings of the National Academy of Sciences, 2004, 101(33): 12294-12299.
CY5是一种含花青（Cyanine）的荧光染料，常用于蛋白、抗体以及小分子化合物的标记，最大激发/发射波长分别为650/670nm[1]。CY5的基本结构是一个带有共轭双键体系的环状化合物，在远红区具有优异的荧光特性，具有较好的光稳定性[2]。CY5通常以其活性酯或叠氮化物的形式存在，这使得它能够通过共价键与蛋白质、核酸等生物分子进行标记[3]。CY5与蛋白的最佳摩尔比为10:1左右[4]。CY5的应用范围非常广泛，不仅适用于生物标记和细胞成像，还可用于核酸检测、蛋白质分析以及药物筛选等领域[5]。本产品分子量为656.81，仅供研究用途，不适用于食品、药品、医疗器械或化妆品。