Berberine (Natural Yellow 18) is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine (Natural Yellow 18) induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine (Natural Yellow 18) has antineoplastic properties[1].
Berberine (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1]. Berberine (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1]. LoVo cells are exposure to Berberine (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].Berberine (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].
Berberine (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].
黄连素（天然黄18）是从中国草药黄连中分离出的一种生物碱，具有抗生素的作用。黄连素能够诱导产生活性氧自由基（ROS），并抑制DNA拓扑异构酶。黄连素具有抗肿瘤的特性[1]。
黄连素（1.25-160 μM；72小时）对四种结肠癌细胞系LoVo、HCT116、SW480和HT-29的增殖具有潜在的抑制作用[1]。黄连素（1.25-160 μM；24-72小时）呈现出对LoVo细胞生长的时间和剂量依赖性抑制作用[1]。将LoVo细胞暴露于10-80 μM浓度的黄连素中处理24小时后，通过流式细胞术分析显示，40 μM浓度的黄连素可导致细胞在G2/M期的积累[1]。黄连素（10-80 μM）可在24小时后抑制cyclin B1、cdc2和cdc25c蛋白表达，尤其在80.0 μM的浓度下表现更为显著[1]。
黄连素（10、30或50 mg/kg/天；经胃灌注；连续10天）能够抑制人类结肠腺癌在体内的生长。黄连素以30和50 mg/kg/天的剂量经胃灌注可分别对裸鼠人类结肠腺癌异种移植物的生长呈现出33.1%和45.3%的抑制率[1]
References:
[1]. Cai Y, et al. Berberine inhibits the growth of human colorectal adenocarcinoma in vitro and in vivo. J Nat Med. 2014 Jan;68(1):53-62.