Cortistatin-14 TFA是一种神经肽，主要存在于大脑皮层和海马体中，可抑制海马CA1神经元的放电活动。
Cas No.:186901-48-4
Sample solution is provided at 25 µL, 10mM.
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Cortistatin-14 TFA, a neuropeptide, is present primarily in the cortex and hippocampus, which inhibits neuronal firing of hippocampal CA1 neurons [1]. Cortistatin-14 TFA inhibits the proliferation of Th1 cells and release of pro-inflammatory cytokines (IL-2 and IFN-γ), while increasing anti-inflammatory signals (IL-10) [2]. Cortistatin-14 TFA has been widely used to regulate the central nervous function of mice and to modulate the sleep/wake rhythm[3].
In vitro, Cortistatin-14 TFA (1µM) treatment for 48 hours significantly inhibited the proliferation of WRO cells[4].
In vivo, Cortistatin-14 TFA treatment via a single intraperitoneal injection at a dose of 500µg/kg significantly increased the survival rate of septic mice within one week and improved cognitive and emotional dysfunction[5]. Ten minutes before intraperitoneal injection of 10mg/kg kainate, 5µl of 10nmol Cortistatin-14 TFA was intracerebroventricularly injected, which significantly reduced the epileptic seizures in rats and inhibited the significant reduction of cortical and hippocampal neurons in rats[6]. A single intraperitoneal injection of 1mg/kg dose of Cortistatin-14 TFA significantly inhibited gastrointestinal transit, microsphere excretion, and fecal particle excretion in mice with colitis[7]. Intracerebroventricular injection of a single dose of 5µg Cortistatin-14 TFA can alleviate depressive-like behaviors in mice and reduce the time spent in inactivity by the mice within 10 minutes[8].
References:
[1] Flood J F, Uezu K, Morley J E. The cortical neuropeptide, cortistatin-14, impairs post-training memory processing[J]. Brain research, 1997, 775(1-2): 250-252.
[2] Markovics A, Szőke É, Sándor K, et al. Comparison of the anti-inflammatory and anti-nociceptive effects of cortistatin-14 and somatostatin-14 in distinct in vitro and in vivo model systems[J]. Journal of Molecular Neuroscience, 2012, 46(1): 40-50.
[3] de Lecea L. Cortistatin—functions in the central nervous system[J]. Molecular and cellular endocrinology, 2008, 286(1-2): 88-95.
[4] Cassoni P, Muccioli G, Marrocco T, et al. Cortistatin-14 inhibits cell proliferation of human thyroid carcinoma cell lines of both follicular and parafollicular origin[J]. Journal of endocrinological investigation, 2002, 25(4): 362-368.
[5] Wen Q, Ding Q, Wang J, et al. Cortistatin‐14 Exerts Neuroprotective Effect Against Microglial Activation, Blood‐brain Barrier Disruption, and Cognitive Impairment in Sepsis‐associated Encephalopathy[J]. Journal of immunology research, 2022, 2022(1): 3334145.
[6] Braun H, Schulz S, Becker A, et al. Protective effects of cortistatin (CST-14) against kainate-induced neurotoxicity in rat brain[J]. Brain research, 1998, 803(1-2): 54-60.
[7] Jiang J H, Jin W D, Peng Y L, et al. The role of Cortistatin-14 in the gastrointestinal motility in mice[J]. Pharmacological Reports, 2018, 70(2): 355-363.
[8] Jiang J H, Peng Y L, Liang X Y, et al. Centrally administered cortistation-14 induces antidepressant-like effects in mice via mediating ghrelin and GABAA receptor signaling pathway[J]. Frontiers in Pharmacology, 2018, 9: 767.
Cortistatin-14 TFA是一种神经肽，主要存在于大脑皮层和海马体中，可抑制海马CA1神经元的放电活动[1]。Cortistatin-14 TFA可抑制Th1细胞增殖及促炎细胞因子（IL-2和IFN-γ）的释放，同时增加抗炎信号（IL-10）[2]。Cortistatin-14 TFA已被广泛用于调节小鼠中枢神经功能及睡眠/觉醒节律[3]。
在体外，1µM的Cortistatin-14 TFA处理48小时可显著抑制WRO细胞的增殖[4]。
在体内，单次腹腔注射500µg/kg的Cortistatin-14 TFA可显著提高脓毒症小鼠一周内的存活率，并改善认知和情绪功能障碍[5]。在腹腔注射10mg/kg红藻氨酸前10分钟，脑室内注射5µl 10nmol的Cortistatin-14 TFA，可显著减少大鼠癫痫发作，并抑制大鼠皮层和海马神经元的显著减少[6]。单次腹腔注射1mg/kg的Cortistatin-14 TFA可显著抑制结肠炎小鼠的胃肠蠕动、微球排泄及粪便颗粒排泄[7]。脑室内注射单剂量5µg的Cortistatin-14 TFA可在10分钟内减轻小鼠抑郁样行为，并减少小鼠的不动时间[8]。
| Cell experiment [1]: | |
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Cell lines |
WRO cells |
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Preparation Method |
WRO cells were grown as a monolayer in RPMI medium with 10% fetal calf serum at 37℃ in a 5% CO2 humidified atmosphere. cells were seeded in quadruplicate in 48-multiwell plates (10000 cells/well) in standard culture medium and allowed to attach for 24h. Cortistatin-14 TFA (0.001, 0.01, 0.1, and 1µM) were then added to the medium and cells were treated up to 96h. Cell viability was evaluated. |
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Reaction Conditions |
0.001, 0.01, 0.1, and 1µM; 96h |
|
Applications |
Cortistatin-14 TFA treatment significantly reduced the cell viability of WRO cells in a dose-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
Kunming Mice |
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Preparation Method |
Male Kunming mice (weighing 20-24g) were housed in sawdust-lined plastic cages and were maintained at 22±1°C (12h light/dark cycle). Drinking water and food were available ad libitum. Establish a mouse model of colitis using dextran sulfate sodium. Mice were fasted for 24h, intraperitoneal injection of Cortistatin-14 TFA (1mg/kg) after five minutes, and were gavaged with 200µl charcoal marker (10%, charcoal suspension in 5% gum arabic solution). The mice were killed 30 minutes after intragastric (i.g.) administration, and the small intestine was removed. The distance traveled by the charcoal marker was measured. |
|
Dosage form |
1mg/kg for once; i.p. |
|
Applications |
Cortistatin-14 TFA treatment significantly inhibited gastrointestinal transit in mice with colitis. |
|
References: |
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| Cas No. | 186901-48-4 | SDF | |
| 分子式 | C83H115F3N20O20S2 | 分子量 | 1834.05 |
| 溶解度 | H2O : 50 mg/mL (Need ultrasonic) | 储存条件 | Store at -20°C |
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| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 545.2 μL | 2.7262 mL | 5.4524 mL |
| 5 mM | 109 μL | 545.2 μL | 1.0905 mL |
| 10 mM | 54.5 μL | 272.6 μL | 545.2 μL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度： mg/ml；
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2.
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