Bacterial Sortase Substrate III, Abz/DNP is a fluorescent quenching substrate (FRET substrate) used to detect bacterial Sortase activity. Bacterial Sortase Substrate III, Abz/DNP consists of a Sortase recognition sequence (LPXTG), an N-terminal fluorescent group Abz, and a C-terminal quencher group DNP, and is commonly used for Sortase A (SrtA) enzyme activity assays and inhibitor screening experiments. After cleavage by Sortase A, Abz is separated from DNP, resulting in the recovery of fluorescence. Cleavage of this substrate can be monitored at Ex/Em=320nm/420nm[1].
References:
[1] Zhang J, et al. Antiinfective therapy with a small molecule inhibitor of Staphylococcus aureus sortase. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13517-22.
Bacterial Sortase Substrate III, Abz/DNP是一种荧光猝灭底物（FRET底物），用于检测细菌分类酶活性。Bacterial Sortase Substrate III, Abz/DNP由一段Sortase识别序列（LPXTG）、N端荧光基团Abz和C端淬灭基团DNP组成，常用于Sortase A（SrtA）酶活性检测和抑制剂筛选实验。经过Sortase A的裂解，Abz与DNP分离，荧光恢复，在Ex/Em=320nm/420nm处可以监测到底物的裂解[1]。