25-Hydroxy VD2-D6 is used as an internal standard for the quantification of 25-Hydroxy VD2 (Ercalcidiol, GC13804) by GC- or LC-MS. 25-Hydroxy VD2 is a metabolite of vitamin D2 that reflects the nutritional status of vitamin D in the body.[1]. 25-Hydroxy VD2-D6 can be used as a tracer. In vivo, 25-Hydroxy VD2 is formed from vitamin D2 by the cytochrome P450 (CYP) isoforms CYP2R1 and CYP27A1, and is bound to vitamin D binding protein (DBP) and albumin in the liver, then 25-Hydroxy VD2 is secreted into the bloodstream is transported to the kidney [2]. The serum level of 25-Hydroxy VD2 have been used as a marker to assess the vitamin D status [3].
References:
[1]. Tugcu, Gulcin, Mohammad Charehsaz, and Ahmet Aydın. "Toxicological evaluation of ergocalciferol, cholecalciferol, and their metabolites by a category approach." Drug and Chemical Toxicology 44.6 (2021): 661-667.
[2]. Bikle, D. "Vitamin D: production, metabolism, and mechanisms of action. MDText. com." Inc.: South Dartmouth, MA, USA (2000).
[3]. Saenger, Amy K., et al. "Quantification of serum 25-hydroxyvitamin D2 and D3using HPLC–Tandem Mass Spectrometry and examination of reference intervals for diagnosis of vitamin D deficiency." American journal of clinical pathology 125.6 (2006): 914-920.
25-Hydroxy VD2-D6 用作通过气相色谱-质谱法（GC-MS）或液相色谱-质谱法（LC-MS）对 25-Hydroxy VD2（Ercalcidiol，GC13804）进行定量分析的内标物。25-Hydroxy VD2 是维生素 D2 的一种代谢产物，能够反映人体内维生素 D 的营养状况 [1]。25-Hydroxy VD2-D6 可用作示踪剂。在体内，维生素 D2 通过细胞色素 P450（CYP）亚型 CYP2R1 和 CYP27A1 转化为 25-Hydroxy VD2，并在肝脏中与维生素 D 结合蛋白（DBP）和白蛋白结合，随后 25-Hydroxy VD2 从肝脏分泌到血液中，并被运输至肾脏[2]。血清中 25-Hydroxy VD2 的水平已被用作评估维生素 D 状况的指标[3]。