Ganoderic acid Y是HMG-CoA还原酶抑制剂和α-葡萄糖苷酶（α-glucosidase）抑制剂，其IC50值分别为8.6μM和170μM。
Cas No.:86377-52-8
Sample solution is provided at 25 µL, 10mM.
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Ganoderic acid Y is HMG-CoA Reductase inhibitor and α-glucosidase inhibitor with IC50s of 8.6μM and 170μM, respectively [1,2]. Ganoderic acid Y can be used in cancer research.
In vitro，Ganoderic acid Y inhibited enterovirus 71 (EV71) replication through blocking EV71 uncoating[3]. Ganoderic acid Y inhibited the cell viabilities of K562 and PC-3 cell lines with IC50s of 17.5 and 179.8μM [2]. Ganoderic acid Y inhibited cell viability of H460 in concentration-dependent manner, with IC₅₀ of 22.4μM [4].
References:
[1]. Chen, Xian-Qiang, et al. "Lanostane triterpenes from the mushroom Ganoderma resinaceum and their inhibitory activities against α-glucosidase." Phytochemistry 149 (2018): 103-115.
[2]. Wang, Kai, et al. "Lanostane triterpenes from the Tibetan medicinal mushroom Ganoderma leucocontextum and their inhibitory effects on HMG-CoA reductase and α-glucosidase." Journal of Natural Products 78.8 (2015): 1977-1989.
[3]. Zhang, Wenjing, et al. "Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection." Biochemical and biophysical research communications 449.3 (2014): 307-312.
[4]. Du, Guo-Hua, et al. "Anti-tumor target prediction and activity verification of Ganoderma lucidum triterpenoids." China Journal of Chinese Materia Medica 42.3 (2017): 517-522.
Ganoderic acid Y是HMG-CoA还原酶抑制剂和α-葡萄糖苷酶（α-glucosidase）抑制剂，其IC50值分别为8.6μM和170μM[1,2]。Ganoderic acid Y可用于癌症研究。
在体外研究中，Ganoderic acid Y通过阻止肠道病毒71型（EV71）的脱壳而抑制其复制[3]。Ganoderic acid Y可以抑制K562和PC-3细胞系的细胞活力，其IC50值分别为17.5和179.8μM[2]。Ganoderic acid Y以浓度依赖的方式抑制H460细胞的活力，其IC50为22.4μM[4]。
| Cell experiment [1]: | |
Cell lines | Lung cancer cell line H460 |
Preparation Method | The cell suspension of tumor cells was inoculated at a certain concentration into a 96-well culture plate and incubated in a 37℃, 5% CO2 incubator for 24 hours. The working solution of Ganoderic acid Y was added at a final concentration of 0.1, 1, 10, 100μM (4 duplicate wells for each concentration). After 72 hours of treatment, the culture medium was discarded, detect cell viability using the MTT method. |
Reaction Conditions | 0.1, 1, 10, 100μM; 72 hours |
Applications | Ganoderic acid Y inhibited cell viability of H460, with IC₅₀ of 22.4μM. |
References: | |
| Cas No. | 86377-52-8 | SDF | |
| 别名 | 灵芝酸Y | ||
| 分子式 | C30H46O3 | 分子量 | 454.68 |
| 溶解度 | 储存条件 | Store at -20°C, protect from light | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1993 mL | 10.9967 mL | 21.9935 mL |
| 5 mM | 439.9 μL | 2.1993 mL | 4.3987 mL |
| 10 mM | 219.9 μL | 1.0997 mL | 2.1993 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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