Z-Leu-Arg-AMC is a 4-methylcoumarinyl-7-amide (AMC) leucine derivative with carboxybenzoyl (Z). Z-Leu-Arg-AMC can be cleaved by cathepsin B, L, and S, and is mainly used to detect the enzyme activity of cysteine proteases such as cathepsin B[1][2][3].
References:
[1] Yoon MC, Phan V, Podvin S, Mosier C, O'Donoghue AJ, Hook V. Distinct Cleavage Properties of Cathepsin B Compared to Cysteine Cathepsins Enable the Design and Validation of a Specific Substrate for Cathepsin B over a Broad pH Range. Biochemistry. 2023;62(15):2289-2300.
[2] Song Y, Fan H, Anderson MJ, et al. Electrochemical Activity Assay for Protease Analysis Using Carbon Nanofiber Nanoelectrode Arrays. Anal Chem. 2019;91(6):3971-3979.
[3]BRENDA Enzyme Database. Cathepsin L (EC 3.4.22.15) substrate information. Technische Universität Braunschweig. Accessed March 10, 2026. https://www.brenda-enzymes.org
Z-Leu-Arg-AMC是4-甲基香豆素基-7-酰胺（AMC）亮氨酸衍生物，带有羧基苯甲酰（Z）。Z-Leu-Arg-AMC可以被组织蛋白酶B、L和S切割，主要用于检测组织蛋白酶B等半胱氨酸蛋白酶的酶活性[1][2][3]。