GlpBio

H-Tyr-His-OH

目录号: GA22973纯度: >98.00%
H-Tyr-His-OH 保持为组氨酸定量分析的内标，并在 8.06 分钟时洗脱。
H-Tyr-His-OH
Cas No.: 3788-44-1
规格价格库存数量操作
250mg¥3,832.00现货
1
1g¥9,895.00现货
1
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文献被引

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    641, 529–536 (2025)
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    610, 366–372 (2022)
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    187(9):2288-2304 (2024)
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    183(7):1867-1883 (2020)
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产品描述 Description

H-Tyr-His-OH was maintained as an internal standard for histidine quantitative analysis and eluted at 8.06 min. In addition, since it does not exist in tissues as an endogenous peptide, it is easy to form positive ions through ESI ionization, with good recovery.[1,2]

References:
[1]: Aldini G, Orioli M,et,al. Profiling histidine-containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry. J Mass Spectrom. 2004 Dec;39(12):1417-28. doi: 10.1002/jms.696. PMID: 15578740.
[2]: Orioli M, Aldini G, et,al. LC-ESI-MS/MS determination of 4-hydroxy-trans-2-nonenal Michael adducts with cysteine and histidine-containing peptides as early markers of oxidative stress in excitable tissues. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 15;827(1):109-18. doi: 10.1016/j.jchromb.2005.04.025. PMID: 15916929.

H-Tyr-His-OH 作为组氨酸定量分析的内标，并在 8.06 分钟时洗脱。此外，由于它不作为内源性肽存在于组织中，通过ESI电离很容易形成正离子，回收率好。[1,2]

实验参考方法 Experimental Reference Method

Quantitative analysis experiment [1]:

Samples Preparation

Calibration samples were prepared by spiking lung homogenate (1 : 3, w/v, in PBS) with each working solution.

Preparation Method

Samples (plasma or tissue homogenates from male rats) were prepared by protein precipitation with HClO(4) (1 : 1, v/v) containing H-Tyr-His-OH as internal standard.

Reaction Conditions

Using 0.1% HFBA as ion-pairing agent: the histidine-containing dipeptides eluted at 4.54 min (CAR), 4.64 min (ANS, HCAR) and 5.06 min (BAL), with an excellent bell shape. H-Tyr-His-OH was chosen as IS for quantitative analysis.

Applications

The mapping profile( H-Tyr-His-OH as internal standard) in rat tissue gave the following results: the highest concentrations of CAR and ANS were found in skeletal muscles (soleus, gastrocnemius, tibialis), followed by the heart, cerebellum and brain (ANS below the LOQ). HCAR was found only in the brain and cerebellum. No histidine-containing dipeptides were detectable in plasma, liver, kidney and lung.

References:

[1]. Aldini G, Orioli M,et,al. Profiling histidine-containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry. J Mass Spectrom. 2004 Dec;39(12):1417-28. doi: 10.1002/jms.696. PMID: 15578740.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
3788-44-1
分子式
C15H18N4O4
分子量
318.33 g/mol
溶解性
Soluble in DMSO
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol