Tos-Gly-Pro-Arg-AMC . HCl is a fluorescent substrate for thrombin, which is widely used to detect serine protease activity. Its excitation and emission wavelengths are 380nm and 460nm, respectively.
The core structure of Tos-Gly-Pro-Arg-AMC . HCl contains a fluorescent group 7-amino-4-methylcoumarin (AMC). When the substrate is hydrolyzed by protease, AMC is released, generating a strong fluorescent signal (excitation wavelength 380nm, emission wavelength 460nm). The Gly-Pro-Arg sequence is a specific recognition site for thrombin, so this substrate is highly selective for thrombin and is also suitable for the study of other serine proteases (such as trypsin, kallikrein, etc.). Tos-Gly-Pro-Arg-AMC . HCl can also be used for high-throughput screening of thrombin inhibitors or for detecting thrombin activity in blood samples to assist in the diagnosis of coagulation abnormalities[1][2].
References:
[1]. Corvo I, Cancela M, Cappetta M, et al. The major cathepsin L secreted by the invasive juvenile Fasciola hepatica prefers proline in the S2 subsite and can cleave collagen[J]. Molecular and biochemical parasitology, 2009, 167(1): 41-47.
[2]. Wirsching F, Luge C, Schwienhorst A. Modular design of a novel chimeric protein with combined thrombin inhibitory activity and plasminogen-activating potential[J]. Molecular genetics and metabolism, 2002, 75(3): 250-259.
Tos-Gly-Pro-Arg-AMC . HCl是一种凝血酶荧光底物，广泛用于检测丝氨酸蛋白酶活性，其激发波长和发射波长分别为380nm和460nm。
Tos-Gly-Pro-Arg-AMC . HCl的核心结构包含一个荧光基团7-氨基-4-甲基香豆素（AMC），当底物被蛋白酶水解时，AMC被释放，产生强荧光信号（激发波长380nm，发射波长460nm）。Gly-Pro-Arg序列是凝血酶的特异性识别位点，因此该底物对凝血酶具有高度选择性，同时也适用于其他丝氨酸蛋白酶（如胰蛋白酶、激肽释放酶等）的研究。Tos-Gly-Pro-Arg-AMC . HCl还可用于高通量筛选凝血酶抑制剂，或检测血液样本中凝血酶的活性以辅助诊断凝血功能异常[1][2]。