H-Gly-Pro-Hyp-OH是一种口服胶原三肽，具有较高的酶稳定性和肠道渗透性。
Cas No.:2239-67-0
Sample solution is provided at 25 µL, 10mM.
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H-Gly-Pro-Hyp-OH is an oral collagen tripeptide with high enzyme stability and intestinal permeability [1]. H-Gly-Pro-Hyp-OH is a moderately competitive inhibitor of dipeptidylpeptidase-IV with a Ki value of 4.5mM[2]. H-Gly-Pro-Hyp-OH has unique specificity for platelet Gp VI and mediates the activation of platelets by collagen [3]. H-Gly-Pro-Hyp-OH treatment inhibits the excessive growth of S. mutans and promotes the dominant growth of S. oralis Dominance [4]. H-Gly-Pro-Hyp-OH has been widely used to improve skin conditions and regulate the central nervous system [5].
In vivo, H-Gly-Pro-Hyp-OH treatment via oral administration at a dose of 31.36mg/kg/day for 6 weeks increased the content of elastin in the skin of aged mice exposed to light, and alleviated the damage to the skin structure on the back caused by chronic ultraviolet radiation[6].
References:
[1] Sontakke S B, Jung J, Piao Z, et al. Orally available collagen tripeptide: enzymatic stability, intestinal permeability, and absorption of Gly-Pro-Hyp and Pro-Hyp[J]. Journal of agricultural and food chemistry, 2016, 64(38): 7127-7133.
[2] Hatanaka T, Kawakami K, Uraji M. Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity[J]. Journal of enzyme inhibition and medicinal chemistry, 2014, 29(6): 823-828.
[3] Knight C G, Morton L F, Onley D J, et al. Collagen–platelet interaction: Gly-Pro-Hyp is uniquely specific for platelet Gp VI and mediates platelet activation by collagen[J]. Cardiovascular research, 1999, 41(2): 450-457.
[4] Jung H Y, Cai J N, Yoo S C, et al. Collagen peptide in a combinatorial treatment with Lactobacillus rhamnosus inhibits the cariogenic properties of Streptococcus mutans: An in vitro study[J]. International Journal of Molecular Sciences, 2022, 23(3): 1860.
[5] Hatanaka T, Kawakami K, Uraji M. Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity[J]. Journal of enzyme inhibition and medicinal chemistry, 2014, 29(6): 823-828.
[6] Yang D, Liu Q, Xu Q, et al. Effects of collagen hydrolysates on UV-induced photoaging mice: Gly-Pro-Hyp as a potent anti-photoaging peptide[J]. Food & function, 2024, 15(6): 3008-3022.
H-Gly-Pro-Hyp-OH是一种口服胶原三肽，具有较高的酶稳定性和肠道渗透性[1]。H-Gly-Pro-Hyp-OH是dipeptidylpeptidase-IV的中等强度竞争性抑制剂，Ki值为4.5mM[2]。H-Gly-Pro-Hyp-OH对血小板Gp VI具有独特的特异性，并介导胶原诱导的血小板活化[3]。H-Gly-Pro-Hyp-OH处理可抑制S. mutans的过度生长，并促进S. oralis的优势生长[4]。H-Gly-Pro-Hyp-OH已被广泛用于改善皮肤状况和调节中枢神经系统[5]。
在体内，每日口服31.36mg/kg剂量的H-Gly-Pro-Hyp-OH，连续6周，增加了受光照的衰老小鼠皮肤中弹性蛋白的含量，并减轻了慢性紫外线辐射对背部皮肤结构的损伤[6]。
| Animal experiment [1]: | |
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Animal models |
Female Kunming mice |
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Preparation Method |
Female Kunming mice (4 weeks old), weighing 18-22g were kept in temperature-controlled (21°C-25°C) and humidity-maintained (50%-60%) rooms with a 12-h light/dark cycle. Water and food were provided freely. All mice were acclimatized for a week before the experiments. After acclimation, the mice were randomly divided into 3 groups (n=10) as follows: (i) normal control group, UV-unexposed, (ii) UV group, (iii) H-Gly-Pro-Hyp-OH group (31.36mg/kg/day; p.o.). Except for the normal control group, the dorsal skins of mice in the other groups were irradiated with four UVA and two UVB lamps three times a week for a total of six weeks. Before each irradiation, the dorsal skin hair was removed using a shaver, followed by applying hair removal cream to the skin for thorough depilation. Appropriate cages were used to prevent mice from moving during irradiation. The irradiation intensity was measured with a radiometer to determine the minimal erythema dose (MED) and the distance between the skins and lamps was 30cm. The irradiation dose was 0.5MED/time in the first week, 1.0MED/time in the second week, and was escalated by 0.5MED/time per week until 3.0MED/time in the sixth week. All the oral administrations began 30min before irradiation. At the end of the photoaging test, the mice were sacrificed. Appropriate dorsal skin tissues were taken for histological staining. |
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Dosage form |
31.36mg/kg/day for 6 weeks; p.o. |
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Applications |
H-Gly-Pro-Hyp-OH treatment increased the content of elastin in the skin of aged mice exposed to UV. |
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References: |
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| Cas No. | 2239-67-0 | SDF | |
| 别名 | 三肽-29 | ||
| 分子式 | C12H19N3O5 | 分子量 | 285.3 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.5051 mL | 17.5254 mL | 35.0508 mL |
| 5 mM | 701 μL | 3.5051 mL | 7.0102 mL |
| 10 mM | 350.5 μL | 1.7525 mL | 3.5051 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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