H-Gln-βNA . HCl is a specific fluorescent substrate for glutaminyl cyclase, with the excitation wavelength of 320nm and emission wavelength of 410nm[1]. H-Gln-βNA . HCl can be used to detect the activity-dependent secretion of glutaminyl cyclase in the secretory products of chromaffin cells[2]. H-Gln-βNA . HCl can be used to evaluate the glutaminyl cyclase inhibitors related to metal chelating agents and imidazole derivatives[3].
References:
[1] Koch B, Kolenko P, Buchholz M, et al. Crystal structures of glutaminyl cyclases (QCs) from Drosophila melanogaster reveal active site conservation between insect and mammalian QCs[J]. Biochemistry, 2012, 51(37): 7383-7392.
[2] Cynis H, Funkelstein L, Toneff T, et al. Pyroglutamate-amyloid-β and glutaminyl cyclase are colocalized with amyloid-β in secretory vesicles and undergo activity-dependent, regulated secretion[J]. Neurodegenerative Diseases, 2014, 14(2): 85-97.
[3] Huang K F, Hsu H L, Karim S, et al. Structural and functional analyses of a glutaminyl cyclase from Ixodes scapularis reveal metal-independent catalysis and inhibitor binding[J]. Biological Crystallography, 2014, 70(3): 789-801.
H-Gln-βNA . HCl是一种用于谷氨酰胺酰环化酶的特异性荧光底物，激发波长为320nm，发射波长为410nm[1]。H-Gln-βNA . HCl可用于检测嗜铬细胞分泌产物中谷氨酰胺酰环化酶的活性依赖性分泌[2]。H-Gln-βNA . HCl可用于评估与金属螯合剂和咪唑衍生物相关的谷氨酰胺酰环化酶抑制剂[3]。